关键词: 荞麦, 种子消毒, 愈伤组织, 植物生长调节物质

Abstract: In this paper, it were studied that effect of H 2O 2 and HgCl₂ solution to sterilization of common buckwheat seeds and phytohormone affect to induction and propagation of callus. Contamination rate were from 0 to 6.2% and germination rate were above 92% after seeds of 5 varieties (Yu3-3, Limadao, Pujiang, Longshan and Fuyuanhonghua) were sterilized with 10% H 2O 2 solution for 150min.It was applicable to seeds of 3 varieties (8621, B-Dali and America) to sterilize with 0.1% HgCl₂ solution for 12min, contamination rate of seeds were 0 and germination rate were from 56.1% to 68.7%. On media supplemented with 0.5~4.0mg/L 2, 4-D, cotyledon and hypocotyl explants from sterilized seedlings were dedifferentiated and calli were formed. Use of BA was very importent in medium containing 2, 4-D, it was helpful for propagation of callus and was unfavourable for differentiation of roots.

Key words: Buckwheat, Seed sterilization, Callus, Plant growth regulator