异戊烯基转移酶基因转化胡卢巴的研究

植物研究 ›› 2000, Vol. 20 ›› Issue (4): 420-426.

异戊烯基转移酶基因转化胡卢巴的研究

黄永红¹, 尤凤丽¹, 孔若东¹, 王付德¹, 汪清胤², 付桂荣², 黄永芬²

1. 大庆高等专科学校, 大庆 163712;
2. 哈尔滨师范大学, 哈尔滨 150080

收稿日期:1999-07-29 出版日期:2000-12-15 发布日期:2016-06-14
作者简介:黄永红(1960-),女,副教授。主要从事:遗传学教学和遗传工程研究。
基金资助:
本论文为大庆石油管理局科技资金资助项目和蔡火石生命科学资金部分资助项目

STUDY ON SAUR-IPT GENE TRANSFORMED TRIGONELLA. FOONELLA. FOENUM. GRAECUM

HUANG Yong-Hong¹, YOU Feng-Li¹, KONG Ruo-Dong¹, WANG Fu-De¹, WANG Qing-Yin², FU Gui-Rong², HUANG Yong-Fen²

1. Daqing high training school, Daqing, Post code 163712;
2. Harbin normal university, Harbin, Post code 150080

Received:1999-07-29 Online:2000-12-15 Published:2016-06-14

摘要/Abstract

摘要： 将含有生长素诱导的小分子RNA启动子与异戊烯基转移酶基因的融合基因(pSAUR-ipt)的Ti质粒作为供体DNA,通过花粉管通道法导入胡卢巴中。D₁、D₂代植株经Southern杂交检测,有杂交带出现,获得了转基因胡卢巴。对转基因胡卢巴D₃、D₄和D₅代进行了生理生化指标及田间性状分析,发现转基因胡卢巴与CK相比有植株略矮、分枝数增多、双角数增多等表型变化,D₄代叶绿素含量、半乳甘露聚糖含量增加,D₅代可溶性蛋白和细胞分裂素含量增加,它们都与产量呈正相关性。从而证明了pSAUR-ipt基因已导入胡卢巴中。

关键词: 胡卢巴, Ti质粒, pSAU R-ipt基因, 花粉管通道法

Abstract: By means of the pollen tube pathway, the Ti plasmid which carrying the SAUR-ipt gene was transferred into Trigonella. Foonella. Foenum. graecum. We tested D₁ generation plants with DNA southern blot hybridization and got five transformed plants. Analyzing the changes of morphology physiology and field characters of D₃, D₄ and D₅ generation transformed plants, we found that the plants were dwarf slightly, amount of branch and double-legume increased, the shape of leaf became narrow, the content of chlorophyll and plant pastern increased in D₄ generation, and solvable protein and ZRs increased in D₅ generation. They are all reveal positive pertinence for the yield. Therefore, it is proved that SAUR-ipt gene already transferred into Trigonella. foonella. foenum. graecum.

Key words: Trigonella. foonella. foenum. graecum, Ti plasmid, ipt gene, pollen tube pathway

黄永红, 尤凤丽, 孔若东, 王付德, 汪清胤, 付桂荣, 黄永芬. 异戊烯基转移酶基因转化胡卢巴的研究[J]. 植物研究, 2000, 20(4): 420-426.

HUANG Yong-Hong, YOU Feng-Li, KONG Ruo-Dong, WANG Fu-De, WANG Qing-Yin, FU Gui-Rong, HUANG Yong-Fen. STUDY ON SAUR-IPT GENE TRANSFORMED TRIGONELLA. FOONELLA. FOENUM. GRAECUM[J]. Bulletin of Botanical Research, 2000, 20(4): 420-426.