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植物研究 ›› 2000, Vol. 20 ›› Issue (4): 416-419.

• 论文 • 上一篇    下一篇

荞麦愈伤组织分化与过氧化物酶活性及其同工酶关系的研究

郝建平, 裴雁曦, 曲运波   

  1. 山西大学生命科学系, 太原 030006
  • 收稿日期:1999-02-04 出版日期:2000-12-15 发布日期:2016-06-14
  • 作者简介:郝建平(1959-),男,山西大学生命科学系副教授。研究方向为植物细胞工程。

STUDY ON PEROXIDASE ACTIVITY AND ITS ISOENZYMES IN CALLUS DIFFERENTIATION OF COMMON BUCKWHEAT

HAO Jian-ping, PEI Yan-xi, QU Yun-bo   

  1. Department of Life Science, Shanxi University, Taiyuan 030006
  • Received:1999-02-04 Online:2000-12-15 Published:2016-06-14
  • Supported by:
    The project is f inanced by Shanxi Natural Science Funds(961020)

摘要: 在附加不同外源激素的培养基中,龙山荞的子叶愈伤组织表现出不同的分化状态:(1)不分化;(2)分化根;(3)分化芽;(4)分化全苗。四种愈伤组织的过氧化物酶活性的相对比值为1:1.47:5.95:7.78,并且过氧化物酶同工酶谱也存在明显差异。酶带4'为分化愈伤组织所特有;酶带2'为未分化和分化愈伤组织所共有;在分化根和未分化愈伤组织中缺少酶带1';未分化愈伤组织比分化愈伤组织多出酶带3'。

关键词: 荞麦, 组织培养, 过氧化物酶, 过氧化物酶同工酶

Abstract: On various mediums with different variety and level of phytohormone, cotyledon callus of Longshan common buckwheat showed different differentiation states: (1) no differentiation took place; (2) root differentiation; (3) bud differentiation and (4) plantlet differentiation. The ratio of peroxidase activity in 4 kinds of calli is 1:1.47:5.95:7.78. There appeared also significant difference among their zymogram of peroxidase isoenzyme. Band 4' in zymogram is characteristic for the differentiated callus; band 2' is common for the undifferentiated and the differentiated; band 1' is of shortage for the differentiated root and the undifferentiated; band 3' is an extra for the undifferentiated.

Key words: Common buckwheat, Tissue culture, Peroxidase, Peroxidase isoenzyme