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植物研究 ›› 2000, Vol. 20 ›› Issue (3): 324-327.

• 论文 • 上一篇    下一篇

干旱胁迫对红松幼苗针叶超微结构的影响

李晶1, 马书荣2, 阎秀峰2, 祖元刚2   

  1. 1. 东北农业大学, 哈尔滨 150030;
    2. 东北林业大学, 哈尔滨 150040
  • 收稿日期:2000-05-09 出版日期:2000-09-15 发布日期:2016-06-14
  • 作者简介:李晶(1970-),女,博士,从事植物生理生态学。
  • 基金资助:
    黑龙江省杰出青年基金资助项目

EFFECTION OF WATER STRESS ON ULTRASTRUCTURE IN LEAVES OF KOREAN PINE SEEDLING

LI Jing1, MA Shu-rong2, YAN Xiu-feng2, ZU Yuan-gang2   

  1. 1. Northeast Agriculture University, Harbin 150030;
    2. Northeast Forestry University, Harbin 150040
  • Received:2000-05-09 Online:2000-09-15 Published:2016-06-14

摘要: 在土壤逐渐失水和PEG溶液模拟的两种不同形式的干旱胁迫下,红松幼苗针叶细胞中叶绿体和线粒体超微结构的变化呈现出显著的差异。在土壤干旱胁迫下,叶绿体的片层结构发生严重的扭曲并在中央部分形成电子密度较高的黑色团块物质。PEG模拟的干旱胁迫下,叶绿体肿胀,空泡化明显,但在叶绿体的中央没有黑色团块物质的形成。在土壤干旱胁迫后,线粒体的膜结构仍然完整清晰,基质比胁迫前更为浓厚,而PEG溶液的干旱胁迫下,线粒体的数量增多,嵴明显减少,基质变得十分稀薄。可见两种不同方式的干旱胁迫处理下,细胞结构的损伤机制及损伤后产生的生理意义并不完全相同。

关键词: 红松幼苗, 干旱胁迫, 叶绿体, 线粒体

Abstract: The changes of ultrastructure in leaves of Korean pine (Pinus koraiensis Sieb. et. Zucc) are different under soil drought stress and PEG drought stress. Under soil drought stress lamella structure of chloroplast wrenched obviously and there was a black area, which had high electron density in the middle of chloroplast. Under PEG drought stress chloroplasts were swollen but no black area. Under soil drought stress the membrane of mitochondria remained clear and intact. mitochondrial matrix became much more darker and thicker. While under PEG drought stress the quantity of mitochondrion increased but the numbers of mitochondria decreased and mitochondrial matrix became thinner. It can be seen that under the two methods of drought stress the mechanism of damage and its physiological meaning maybe different too.

Key words: Korean pine (Pinus koraiensis) seedling, drought stress, chloroplast, mitochondrion