“凤丹”牡丹花丝愈伤组织诱导及分化研究

doi:10.7525/j.issn.1673-5102.2020.04.005

植物研究 ›› 2020, Vol. 40 ›› Issue (4): 514-522.doi: 10.7525/j.issn.1673-5102.2020.04.005

“凤丹”牡丹花丝愈伤组织诱导及分化研究

杜一鸣¹, 钟原¹, 尚宏芹², 成仿云¹

1. 北京林业大学林木分子设计育种高精尖创新中心, 牡丹国际研究院, 国家花卉工程技术中心, 园林学院, 北京 100083;
2. 菏泽学院农业与生物工程学院, 菏泽 274015

收稿日期:2019-12-15 出版日期:2020-07-05 发布日期:2020-06-12
通讯作者: 成仿云,E-mail:chengfy8@263.net E-mail:chengfy8@263.net
作者简介:杜一鸣(1990-),女,博士研究生,主要从事花卉种质创新与育种方面的研究。
基金资助:
北京园林植物高效繁殖技术研究（2019-KJC-02-10-1）；北京林业大学世界一流学科建设和特色发展指导基金（2019XKJS0324）

Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’

DU Yi-Ming¹, ZHONG Yuan¹, SHANG Hong-Qin², CHENG Fang-Yun¹

1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Peony International Institute, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083;
2. College of Agricultural and Biological Engineering, Heze University, Heze 274015

Received:2019-12-15 Online:2020-07-05 Published:2020-06-12
Supported by:
Research of Efficient Propagation Technology of Ornamental Plants in Beijing(2019-KJC-02-10-1);World-Class Discipline Construction and Characteristic Development Guidance Funds for Beijing Forestry University(2019XKJS0324)

摘要/Abstract

摘要： 以"凤丹"花丝为外植体，探究了外植体发育时期、基本培养基和植物生长调节剂（plant growth regulators，PGRs）对愈伤组织诱导、增殖和分化的影响，并对不同类型的愈伤组织进行了形态组织学观察。结果表明：花蕾直径为13~19mm时、半透明或白色的幼嫩花丝更适宜愈伤组织的诱导；愈伤组织诱导的最适培养基为SH添加2，4-D 1 mg·L^-1、NAA 2 mg·L^-1及BA 0.2 mg·L^-1，最高诱导率和诱导量分别为99.12%和5.68 g；愈伤组织增殖的最适培养基为SH添加2，4-D 0.5 mg·L^-1、NAA 0.5 mg·L^-1及BA 0.25 mg·L^-1，增殖率为3.86；花丝愈伤组织根据形态结构可分为三类，Ⅰ类愈伤组织具有典型的胚性愈伤组织特征，可进一步分化产生体胚；Ⅱ类愈伤组织和Ⅲ类愈伤组织内部呈现大量维管组织分化，其中Ⅱ类愈伤组织中观察到了不定芽的分化，Ⅲ类愈伤组织未见分化。上述结果表明"凤丹"花丝具有重要再生潜力，是一种良好的新型外植体，对研究建立牡丹体外再生体系具有重要价值。

关键词: “凤丹”, 花丝, 愈伤组织

Abstract: With the filaments of Paeonia ostii as explants, the effect of developmental stage of explant, basal media and PGRs on callus induction, proliferation and differentiation were investigated, and the morph-histological observation of different types of callus was performed. The translucent and white filaments from flower bud of 13-19 mm in diameter is optimal for callus induction. The optimal callus induction medium was SH containing 1 mg·L^-1 2,4-D, 2 mg·L^-1 NAA and 0.2 mg·L^-1 BA. The optimal proliferation medium was SH containing 0.5 mg·L^-1 2,4-D, 0.5 mg·L^-1 NAA and 0.25 mg·L^-1 BA, and the proliferation rate was 3.86. The filaments callus can be divided into three types according to their morphological and microstructural characteristic, the type Ⅰ callus presented typical embryogenic callus characteristics, and somatic embryogenesis was observed from this kind of callus; there were plenty of vascular tissues formed in the type Ⅱ and type Ⅲ callus, and shoot organogenesis was observed from the type Ⅱ callus, but no morphogenesis from the type Ⅲ callus. These results suggested that the filaments have important regeneration potential as a good novel explant source, which have important value in establishing in vitro regeneration system of tree peony.

Key words: Paeonia ostii ‘Feng Dan’, filament, callus

中图分类号:

S685.11

杜一鸣, 钟原, 尚宏芹, 成仿云. “凤丹”牡丹花丝愈伤组织诱导及分化研究[J]. 植物研究, 2020, 40(4): 514-522.

DU Yi-Ming, ZHONG Yuan, SHANG Hong-Qin, CHENG Fang-Yun. Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’[J]. Bulletin of Botanical Research, 2020, 40(4): 514-522.

参考文献

[1] Li S S,Yuan R Y,Chen L G,et al.Systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree peony(Paeonia sectionmoutan DC.) cultivars by GC-MS[J].Food Chemistry,2015,173:133-140.
[2] Yu S Y,Du S B,Yuan J H,et al.Fatty acid profile in the seeds and seed tissues of Paeonia L.species as new oil plant resources[J].Scientific Reports,2016,6(1):26944.
[3] 王新."凤丹"牡丹离体快繁技术研究[D].北京:北京林业大学,2016.Wang X.In vitro rapid propagation of Paeonia ostii ‘Feng Dan’[D].Beijing:Beijing Forestry University,2016.
[4] 文书生,成仿云,钟原,等."正午"牡丹微繁殖体系的建立[J].植物科学学报,2016,34(1):143-150.Wen S S,CHENG F Y,ZHONG Y,et al.Protocol for the micropropagation of tree peony(Paeonia×lemoinei ‘High Noon’)[J].Plant Science Journal,2016,34(1):143-150.
[5] 徐莉,成仿云,钟原.牡丹胚培养快速成苗技术研究[J].植物研究,2017,37(5):690-699.Xu L,Cheng F Y,Zhong Y.Study on rapid seedling-raising technology of tree peony embryo culture[J].Bulletin of Botanical Research,2017,37(5):690-699.
[6] 周秀梅,成仿云,钟原,等.紫斑牡丹"书生捧墨"的体胚诱导与发生[J].北京林业大学学报,2009,31(2):151-154.Zhou X M,Cheng F Y,Zhong Y,et al.Inducement and development of somatic embryos in Paeonia rockii ‘Shu Sheng Peng Mo’[J].Journal of Beijing Forestry University,2009,31(2):151-154.
[7] 钟原.紫斑牡丹分生结节的诱导与培养[D].北京:北京林业大学,2011.Zhong Y.Induction and culture of meristematic nodules in Paeonia rockii[D].Beijing:Beijing Forestry University,2011.
[8] 朱向涛.凤丹体胚发生及愈伤组织诱导芽分化研究[D].北京:中国林业科学研究院,2010.Zhu X T.Somatic embryos and callus induction and the bud differentiation of Paeonia ostii ‘Fengdan’ Andr[D].Beijing:Chinese Academy of Forestry,2010.
[9] Loyola-Vargas V M,Ochoa-Alejo N.Somatic embryogenesis:fundamental aspects and applications[M].Switzerland:Springer,2016.
[10] 徐莉,成仿云."凤丹"牡丹愈伤组织的诱导研究[C].//中国观赏园艺研究进展2017.成都:中国园艺学会观赏园艺专业委员会,国家花卉工程技术研究中心,2017:574-578.Xu L,Cheng F Y.Preliminary study on callus induction of Paeonia ostii ‘Fengdan’[C].//Advances in ornamental horticulture of China.Chengdu:Professional Committee of Ornamental Horticulture of Chinese Horticultural Society,National flower Engineering Technology Research Center,2017:574-578.
[11] Nakajima I,Matsuta N.Somatic embryogenesis from filaments of Vitis vinifera L.and Vitis labruscana Bailey[J].Vitis,2003,42(1):53-54.
[12] Stefanello S,Dal Vesco L L,Ducroquet J P H J,et al.Somatic embryogenesis from floral tissues of feijoa(Feijoa sellowiana Berg)[J].Scientia Horticulturae,2005,105(1):117-126.
[13] Zdravković-Korać S,Tubić L,Devrnja N,et al.Somatic embryogenesis from stamen filaments of Aesculus flava Sol.and peroxidase activity during the transition from friable to embryogenic callus[J].Scientia Horticulturae,2019,247:362-372.
[14] George E F,Hall M A,De Klerk G J.Plant propagation by tissue culture:Vol.1.The background hardcover[M].Dordrecht:Springer,2007.
[15] Acanda Y,Prado M J,González M V,et al.Somatic embryogenesis from stamen filaments in grapevine(Vitis vinifera L.cv.Mencía):changes in ploidy level and nuclear DNA content[J].In Vitro Cellular & Developmental Biology-Plant,2013,49(3):276-284.
[16] Park Y S,Lelu-Walter M A,Harvengt L,et al.Initiation of somatic embryogenesis in Pinus banksiana,P.strobus,P.pinaster and P.sylvestris at three laboratories in Canada and France[J].Plant Cell,Tissue and Organ Culture,2006,86(1):87-101.
[17] Nunes S,Marum L,Farinha N,et al.Somatic embryogenesis of hybrid Pinus elliottii var.elliottii×P.caribaea var.hondurensis and ploidy assessment of somatic plants[J].Plant Cell,Tissue and Organ Culture,2018,132(1):71-84.
[18] Andrade G M,Merkle S A.Enhancement of American chestnut somatic seedling production[J].Plant Cell Reports,2005,24(6):326-334.
[19] Lu D,Wei W,Zhou W,et al.Establishment of a somatic embryo regeneration system and expression analysis of somatic embryogenesis-related genes in Chinese chestnut(Castanea mollissima Blume)[J].Plant Cell,Tissue and Organ Culture,2017,130(3):601-616.
[20] 贾文庆,刘会超."凤丹"子叶愈伤组织诱导及分化研究[J].林业实用技术,2009(6):3-5.Jia W Q,Liu H C.Study of callus induction and differentiation from cotyledons of Paeonia ostii ‘Fengdan’[J].Practical Forestry Technology,2009(6):3-5.
[21] Varis S,Klimaszewska K,Aronen T.Somatic embryogenesis and plant regeneration from primordial shoot explants of Picea abies(L.) H.Karst.somatic trees[J].Frontiers in Plant Science,2018,9:1551.
[22] Scherwinski-Pereira J E,Da Silva Guedes R,Da Silva R A,et al.Somatic embryogenesis and plant regeneration in açaí palm(Euterpe oleracea)[J].Plant Cell,Tissue and Organ Culture,2012,109(3):501-508.
[23] Dobrowolska I,Andrade G M,Clapham D,et al.Histological analysis reveals the formation of shoots rather than embryos in regenerating cultures of Eucalyptus globulus[J].Plant Cell,Tissue and Organ Culture,2017,128(2):319-326.
[24] Silvestri C,Cristofori V,Ceccarelli M,et al.Adventitious shoot organogenesis from leaf and petiole explants of European hazelnut[J].Plant Cell,Tissue and Organ Culture,2016,126(1):59-65.

“凤丹”牡丹花丝愈伤组织诱导及分化研究

Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’

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