多孔淀粉负载青蒿素微球的抗肿瘤活性研究

doi:10.7525/j.issn.1673-5102.2019.03.015

植物研究 ›› 2019, Vol. 39 ›› Issue (3): 450-457.doi: 10.7525/j.issn.1673-5102.2019.03.015

多孔淀粉负载青蒿素微球的抗肿瘤活性研究

赵雪, 杨逢建, 钟晨, 张圆圆, 葛云龙, 邓怡平, 隋广超

东北林业大学, 哈尔滨 150040

收稿日期:2019-01-30 出版日期:2019-05-05 发布日期:2019-05-11
通讯作者: 隋广超 E-mail:gcsui@nefu.edu.cn
作者简介:赵雪(1990-),女,博士研究生,主要从事药用植物资源学方向。
基金资助:
黑龙江省林业科技项目（逊克）（HLJXK01）；青蒿素及其衍生物抗肿瘤及其功能机制的研究（2572016AA37）

Antitumor Activity of Artemisinin Microspheres Loaded with Porous Starch

ZHAO Xue, YANG Feng-Jian, ZHONG Chen, ZHANG Yuan-Yuan, GE Yun-Long, DENG Yi-Ping, SUI Guang-Chao

Northeast Forestry University, Harbin 150040

Received:2019-01-30 Online:2019-05-05 Published:2019-05-11
Supported by:
Forestry science and technology project of Heilongjiang province(Xun Ke)(HLJXK01);Study on Anti-tumor and Functional Mechanism of Artemisinin and Its Derivatives(2572016AA37)

摘要/Abstract

摘要： 探讨多孔淀粉负载青蒿素微球（ART-PS）与青蒿素原药（ART）在不同浓度下的抗肿瘤活性，以及分别联合全铁转铁蛋白后对肿瘤细胞的生长抑制作用。在体外实验中，取对数生长期的人肝癌细胞和正常肝细胞接种于96孔板中，不同浓度（0、50、100、150、200 μmol·L^-1）给药处理24 h后，用MTT法分别检测多孔淀粉负载青蒿素微球与青蒿素原药对细胞的生长抑制作用。MTT结果显示，同等处理浓度下，多孔淀粉负载青蒿素微球对肿瘤细胞HepG2和SMMC-7721的抑制效果都高于青蒿素原药，但与盐酸阿霉素相比，都具有较低的细胞毒性，对正常细胞HL7702的毒副作用非常低，结果与分别联合全铁转铁蛋白后对肿瘤细胞的生长抑制作用一致。多孔淀粉负载青蒿素微球对人肝癌细胞的增殖有明显的抑制作用，效果优于青蒿素原药，并对正常肝细胞的毒副作用非常低，为青蒿素在治疗癌症的应用与研究提供了重要的参考依据。

关键词: 青蒿素, 全铁转铁蛋白, 肝癌

Abstract: To investigate the antitumor activity of porous starch loaded artemisinin microspheres(ART-PS) and artemisinin active drugs(ART) at different concentrations, and the growth inhibition of tumor cells after combined with total ferritin, respectively, in the in vitro experiments, human hepatocellular carcinoma cells and normal hepatocytes at logarithmic growth stage were inoculated to 96-well plates at different contents(0, 50, 100, 150 and 200 μmol·L^-1). Concentrations determined by MTT, according to the results of equal treatment, porous starch load on tumor cell HepG2 artemisinin microspheres and the inhibition effect of SMMC-7721 were higher than that of artemisinin technical, but compared with doxorubicin hydrochloride, has low cytotoxicity, on the side effects of normal cells HL7702 is very low, the results associated with respectively after full iron transferrin inhibition for the growth of tumor cells. The porous starch loaded artemisinin microspheres had obvious inhibitory effect on the proliferation of human hepatocellular carcinoma cells, the effect was better than the original artemisinin drugs, and the toxic and side effects on normal hepatocytes were very low, it provides an important reference for the application and research of artemisinin in the treatment of cancer.

Key words: artemisinin, holo-transferrin, liver cancer

中图分类号:

S567.9

赵雪, 杨逢建, 钟晨, 张圆圆, 葛云龙, 邓怡平, 隋广超. 多孔淀粉负载青蒿素微球的抗肿瘤活性研究[J]. 植物研究, 2019, 39(3): 450-457.

ZHAO Xue, YANG Feng-Jian, ZHONG Chen, ZHANG Yuan-Yuan, GE Yun-Long, DENG Yi-Ping, SUI Guang-Chao. Antitumor Activity of Artemisinin Microspheres Loaded with Porous Starch[J]. Bulletin of Botanical Research, 2019, 39(3): 450-457.

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多孔淀粉负载青蒿素微球的抗肿瘤活性研究

Antitumor Activity of Artemisinin Microspheres Loaded with Porous Starch

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