关键词: 转基因, 喜树, UGPase, 木材化学成分, 生长速率

Abstract: Plant UDP-glucose (UDPG) pyrophosphorylase (UGPase) is involved in the production of UDPG, a key metabolite for sucrose and cell wall biosynthesis. It has been shown that UGP can increase the cellulose content of wood. In order to enhance the cellulose content of Camptotheca acuminata Decne, Axgllnum UGP gene had been transferred into C. acuminata. This paper concerned the wood chemical compositions and growth rate of the UGP transgenic C. acuminata, analyzed the data according to the typical broad-leaved pulpwood stander. Result showed that: the contents of holocellulose, cellulose, and lignin were 78.87%, 36.34% and 15.05% respectively, the transformed plants are more fitful for paper making compared with control. From May to November, we checked the height, base diameter and biomass of UGP transgenic plant and control each month, the result showed that: three parameter of transgenic plants raised 78.00%, 236.37% and 322.61% respectively of control. In short, the growth rate of transgenic plant increased obviously. Above all, C. acuminata was a good quality pulpwood, and the UGP, although not increasing the cellulose properties obviously, improved the growth rate and total biomass significantly, and increased the cellulose yield indirectly.

Key words: transgenic, C. acuminata, UGPase, wood chemical compositions, growth rate