白桦锌指蛋白基因的分离及表达分析

doi:10.7525/j.issn.1673-5102.2016.03.012

植物研究 ›› 2016, Vol. 36 ›› Issue (3): 395-400.doi: 10.7525/j.issn.1673-5102.2016.03.012

白桦锌指蛋白基因的分离及表达分析

王宇航, 秦琳琳, 李莉

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2015-12-15 出版日期:2016-05-15 发布日期:2016-05-20
通讯作者: 李莉,E-mail:lili@nefu.edu.cn E-mail:lili@nefu.edu.cn
作者简介:王宇航(1990-),女,硕士研究生,主要从事植物分子生物学研究。
基金资助:
国家自然科学基金(31470676)和科技部"863"课题(2013AA102704-0104)

Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla

WANG Yu-Hang, QIN Lin-Lin, LI Li

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2015-12-15 Online:2016-05-15 Published:2016-05-20
Supported by:
This work was funded by the National Natural Science Foundation of China(Grant No.31470676) and by the Ministry of Science and Technology of China under the "863" program(Grant No.2013AA102704-0104)

摘要/Abstract

摘要： 植物锌指蛋白是成员众多的转录因子家族,在植物的生长发育和对非生物胁迫响应等过程中具有重要作用。根据Cys和His残基的数目和位置将锌指转录因子分为C₂H₂、C₈、C₆、C₃HC₄、C₂HC、C₂HC₅、C₄、CCCH和C₄HC₃九种类型。本实验利用其他物种中已报导的植物逆境胁迫相关的锌指蛋白转录因子基因序列作为信息探针,在白桦基因组数据库中筛选出10个基因,分别是4个C₂H₂型、5个C₃HC₄-RING型和一个CCCH型。通过qRT-PCR技术对筛选的基因在根中的表达模式进行了分析,结果显示BpZFP2、BpZFP3、BpZFP4、BpZFP5和BpZFP8的表达受盐和干旱胁迫的显著诱导,表明这些基因参与了逆境胁迫的应答。

关键词: 白桦, 非生物胁迫, 锌指蛋白转录因子, 表达分析

Abstract: Zinc finger proteins constitute a large family of transcription factors and numerous members of them play diverse roles in many biological processes, including plant growth, development and stress responses. Based on their structural and functional diversities they were classified into nine types including C₂H₂, C₈, C₆, C₃HC₄, C₂HC, C₂HC₅, C₄, CCCH and C₄HC₃. We studied the genes in Betula platyphylla database by the sequences and homology comparison of genes encoding zinc finger proteins involving in stress responses from other organisms. A total of ten genes (designated as BpZFP1 to BpZFP10), of which four were C₂H₂ type, five were C₃HC₄-RING type, one was CCCH type were identified. By quantitative RT-PCR assay, we analyzed the expression patterns of BpZFPs in roots. BpZFP2, BpZFP3, BpZFP4, BpZFP5 and BpZFP8 were induced under high-salt and drought treatment significantly indicating their involvement in stress response in Birch.

Key words: Betula platyphylla, abiotic stress, zinc finger transcription factor, expression analysis

中图分类号:

S792.153

王宇航, 秦琳琳, 李莉. 白桦锌指蛋白基因的分离及表达分析[J]. 植物研究, 2016, 36(3): 395-400.

WANG Yu-Hang, QIN Lin-Lin, LI Li. Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla[J]. Bulletin of Botanical Research, 2016, 36(3): 395-400.

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白桦锌指蛋白基因的分离及表达分析

Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla

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