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植物研究 ›› 2015, Vol. 35 ›› Issue (5): 692-695.doi: 10.7525/j.issn.1673-5102.2015.05.009

• 论文 • 上一篇    下一篇

过表达ThVHAc1对拟南芥V-ATPase各亚基表达的影响

杨桂燕1,2;郭宇聪1;赵玉琳1;张凤娇1;高彩球1*   

  1. 1.东北林业大学林木遗传育种国家重点实验室,哈尔滨 150040;
    2.西北农林科技大学林学院核桃研究中心,杨凌 712100
  • 出版日期:2015-09-15 发布日期:2015-11-20
  • 基金资助:
     

The Expression of VATPase Subunits in Overexpression ThVHAc1 Arabidopsis under Salt Stress

YANG Gui-Yan1,2;GUO Yu-Cong1;ZHAO Yu-Lin1;ZHANG Feng-Jiao1;GAO Cai-Qiu1*   

  1. 1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040;
    2.Laboratory of Walnut Research Center,College of Forestry,Northwest A & F University,Yangling 712100
  • Online:2015-09-15 Published:2015-11-20
  • Supported by:
     

摘要: V-ATPase是多亚基复合蛋白,其c亚基负责V-ATPase的组装及质子通道的形成。本研究拟分析盐胁迫下过表达ThVHAc1基因拟南芥V-ATPase各亚基的表达,探讨过表达外源c亚基对拟南芥V-ATPase全酶响应盐胁迫表达模式的影响。实时荧光定量PCR结果显示,盐胁迫下,过表达外源ThVHAc1拟南芥V-ATPase 28个亚基的表达发生了明显改变,且拟南芥5个c亚基的表达均不同程度的被抑制。表明外源ThVHAc1基因能影响拟南芥V-ATPase各亚基的表达以调节V-ATPase全酶的活性,但各亚基的表达模式与V-ATPase活性非简单对应关系,各亚基互相协调决定V-ATPase活性。

关键词: 刚毛柽柳, 表达分析, c亚基, V-ATPase;ThVHAc1

Abstract: V-ATPase is a muti-subunits protein complex, the c subunit is mainly responsible for V-ATPase assembly and proton channel formation. We measured the expression profiles of V-ATPase subunits in exogenous overexpression ThVHAc1 Arabidopsis under salt by using real time RT-PCR. Twenty-eight V-ATPase subunits were all effected by exogenous ThVHAc1, and five Arabidopsis c subunits were down-regulated expression, indicating that ThVHAc1 affected the V-ATPase activity by altered the expression of V-ATPase all subunits. Between the expression of V-ATPase subunit and V-ATPase activity showed non-monolithic relationship, they were functioned together coordinately on V-ATPase to response to slat stress.

Key words: Tamarix hispida, expression analysis, c subunit, V-ATPase, ThVHAc1

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