金粉蕨属的分子系统学研究——基于5种叶绿体DNA序列片段

doi:10.7525/j.issn.1673-5102.2015.05.005

摘要/Abstract

摘要： 金粉蕨属（Onychium Kaulfuss）隶属广义凤尾蕨科中的凤尾蕨亚科。迄今为止，该属属下分组及种间界定等仍有诸多问题亟待解决。本研究选取5个叶绿体DNA 序列片段（rbcL/atpA/matK/trnL-trnF/trnG-trnR），采用最大似然法（ML）和贝叶斯法（BI）构建金粉蕨属的系统发育树。结果表明：（1）金粉蕨属的9个成员被分置于两大支上。其中野雉尾金粉蕨（Onychium japonicum(Thunberg) Kunze）、西藏金粉蕨（O.tibeticum Ching & S.K.Wu）、木坪金粉蕨（O.moupinense Ching）、湖北金粉蕨（O.moupinense var. ipii(Ching) K.H.Shing）、栗柄金粉蕨（O.japonicum var. lucidum(D.Don) Christ）、黑足金粉蕨（O.cryptogrammoides Christ）、繁羽金粉蕨（O.plumosum Ching）聚为一支；而金粉蕨（O.siliculosum(Desvaux) C.Christensen）和蚀盖金粉蕨（O.tenuifrons Ching）则聚为另一支，可为该属的属下分组提供分子系统学证据；（2）野雉尾金粉蕨与栗柄金粉蕨在系统树中并没有聚在一起，而是被其它类群分割开来，不支持将后者作为野雉尾金粉蕨的变种，建议将栗柄金粉蕨提升为种的等级；（3）系统树上木坪金粉蕨与湖北金粉蕨的样本聚在一个细支上，支持《中国植物志》将湖北金粉蕨作为木坪金粉蕨变种的分类处理；（4）西藏金粉蕨与野雉尾金粉蕨聚在一起，并得到较高的支持，说明两者的关系近缘。

关键词: 金粉蕨属, 系统分类, rbcL/atpA/matK/trnL-trnF/trnG-trnR

Abstract: Onychium Kaulfuss, subfamily Pteridoideae of Pteridaceae, has long been in dispute with respect to its sectional circumscription and interspecific definition. We studied the phylogenetic relationships of Onychium based on five chloroplast DNA sequences(rbcL/atpA/matK/trnL-trnF/trnG-trnR) using Maximum Likelihood(ML) and Bayesian(BI) methods. (1)All species of Onychium were clearly divided into two main groups: Onychium japonicum(Thunberg) Kunze, O.tibeticum Ching & S.K.Wu, O.moupinense Ching, O.moupinense var. ipii(Ching) K. H. Shing, O.japonicum var. lucidum(D. Don) Christ, O.cryptogrammoides Christ and O.plumosum Ching were clustered in a group, while O.siliculosum(Desvaux) C. Christensen and O.tenuifrons Ching were clustered in another group; (2)It provided the molecular evidences for the taxonomy of Onychium at section level. O.japoniucm and O.japoniucm var. lucidum were not united together in the phylogenetic trees, while they were separated by other species, suggesting that O.japoniucm var. lucidum should be treated as a separate species rather than a variety of O.japonicum; (3)O.moupinense and O.moupinense var. ipii were gathered in a strongly supported clade, supporting the taxonomic treatment of O.moupinense var. ipii as a variety of O.moupinense in Flora of China; (4)O.tibeticum and O.japonicum were grouped in a clade with a high bootstrap value with close relation.

Key words: Onychium, phylogenetic analysis, rbcL/atpA/matK/trnL-trnF/trnG-trnR

中图分类号:

窦平1；杨文利2；赵冉冉1；张钢民1*. 金粉蕨属的分子系统学研究——基于5种叶绿体DNA序列片段[J]. 植物研究, 2015, 35(5): 665-671.

DOU Ping1；YANG Wen-Li2；ZHAO Ran-Ran1；ZHANG Gang-Min1*. Phylogenetic Analysis of Onychium Based on Five Chloroplast DNA Sequences[J]. Bulletin of Botanical Research, 2015, 35(5): 665-671.

参考文献

1.Diels L.Polypodiaceae［M］.//Engler A,Prantl K.Die Naturlichen Pflanzenfamilien.Leipzig:Verlag von Wilhelm Engelmann,1902,I(IV):265-282.

2.Ching R C.On the genus Onychium Kaulf.from the far orient［J］.Lingnan Science Journal,1934,13(3):493-501.

3.Ching R C.On natural classification of the family "Polypodiaceae"［J］.Sunyatsenia,1940,5(4):201-268.

4.Pichi-Sermolli R E G.Tentamen pteridophytorum genera in taxonomicum ordinem redigendi［J］.Webbia,1977,31:313-512.

5.秦仁昌，邢公侠.中国植物志［M］.北京：科学出版社,1990,3(1):103-113.

6.Nayar B K,Devi S,Chandr S.Morphology of the genus Onychium in india［J］.J Indian Bot Soc,1972,51:63-72.

7.Nayar B K.A phylogenetic classification of the homosporous ferns［J］.Taxon,1970,19:229-236.

8.Nayar B K,Kaur S.Companion to Beddome's Handbook to the Ferns of British India［J］.The Chronica Botanica,1974.

9.于晶，王全喜，包文美.中国金粉蕨属孢子形态的研究［J］.植物研究,2001b,21(2):227-230.

10.Tryon R M,Tryon A F.Ferns and allied plants with special reference to tropical America［M］.New York:Springer-Verlag,1982,213-354.

11.Tryon R M,Tryon A F,Kramer U.Pteridaceae［M］//Kubitzki K.The Families and Genera of Vascular Plants.Pteridophytes and Gymnosperms.Berlin:Springer-Verlag,1990,1:230-256.

12.杨文利，张钢民.金粉蕨属的系统位置—来自rbcL序列的证据［J］.河北农业大学学报， 2005，28（2）：36-39.

13.Schuettpelz E.A molecular phylogeny of the fern family Pteridaceae:Assessing overall relationships and the affinities of previously unsampled genera［J］.Molecular Phylogenetics and Evolution,2007,44:1172-1185.

14.Christenhusz J M M,Zhang X C,Schneider H.A linear sequence of extant families and genera of lycophytes and ferns［J］.Phytotaxa,2011,19:7-54.

15.张宪春.中国石松类和蕨类植物［M］.北京：北京大学出版社,2011,206-208.

16.Schneider H,He L J,Hennequin S,et al.Towards a natural classification of Pteridaceae:inferring the relationships of enigmatic pteridoid fern species occurring in the SinoHimalaya and AfroMadagascar［J］.Phytotaxa,2013,77(4):49-60.

17.Kummerle J B.Has the genus Onychium any representative in South America［J］.American Fern Journal,1930,20(4):129-138.

18.Pichi-Sermolli R E G.Adumbbatio florae Aetidopicae［J］.Webbia,1963,17(2):299-315.

19.Vaganov A V,Shmakov A I.A system of the genus Onychium Kaulf.(Cryptogrammaceae)［J］.Turczaninowia,2012,15(1):25-27.

20.孔宪需.四川植物志第6卷［M］.成都：四川人民出版社,1988,278-284.

21.Bista M S,Adhikari M K,Rajbhandari K R.Pteridophytes of Nepal［J］.Bull.Dept.of Plant Resources,2002,19:71.

22.秦仁昌，武素功.西藏植物志第1卷［M］.北京：科学出版社,1983,77-78.

23.张光飞.云南植物志第20卷［M］.北京：科学出版社,2006,300-305.

24.Khullar S P,Fraser-Jenkins C R.An illustrated fern flora of West Himalaya［M］.Dehra Dun:Bishen Singh Mahendra Pal Singh,1994,1:506.

25.Zhang G M,Yatskievych G.Flora of China［M］.Beijing:Beijing Science Press & St Louis: Missouri Botanical Garden Press,2013,2:212-216.

26.Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］.Phytochemical Bulletin,1987,19:11-15.

27.Schuettpelz E,Kathleen M P.Fern phylogeny inferres from 400 leptosporangiate species and three plastid genes［J］.Taxon,2007,56(4):1037-1050.

28.Schuettpelz E,Korall P,Pryer K M.Plastid atpA data provide improved support for deep relationships among ferns［J］.Taxon,2006,55:897-906.

29.Taberlet P,Gielly L,Pautau G,et al.Universal primers for amplification of 3 non-coding regions of chloroplast DNA［J］.Plant Molecular Biology,1991,17(5):1105-1109.

30.Kuo L Y,Li F W,Chiou W L,et al.First insights into fern matK phylogeny［J］.Molecular Phylogenetics and Evolution,2011,59:556-566.

31.Nagalingum N S,Schneider H,Pryer K M.Molecular phylogenetic relationships and morphological evolution in the heterosporous fern genus Marsilea［J］.Systematic Botany,2007,32:16-25.

32.Posada D,Crandall K.Modeltest:testing the model of DNA substitution［J］.Bioinformatics,1998,14:817-818.

33.Posada D,Buckley T R.Model selection and model averaging in phylogenetics:Advantages of akaike information criterion and Bayesian approaches over likelihood ratio tests［J］.Systematic Biology,2004,53:793-808.

34.Tamura K,Stecher G,Peterson D,et al.MEGA6:Molecular Evolutionary Genetics Analysis version 6.0［J］.Molecular Biology and Evolution,2013,30:2725-2729.

35.Felsenstein J.Phylogenies from molecular sequences:Inference and reliability［J］.Annual Review of Genetics,1988,22:521-565.

36.Rehnquist F,Huelsenbeck J P.MrBayes 3:Bayesian phylogenetic inference under mixed models［J］.Bioinformatics,2003,19:1572-1574.

37.Brown J M,Lemmon A R.The importance of data partitioning and the utility of Bayes factors in Bayesian phylogenetics［J］.Syst Biol,2007,56:643-655.

38.Fraser-Jenkins C R.Taxonomic Revision of Three Hundred Indian Subcontinental Pteridophytes［M］.Dehra Dun:Bishen Singh Mahendra Pal Singh,2008:89-96.

[1]	姚雪莹, 陈志祥, 王奇志. 变豆菜属11种植物的叶表皮微形态特征研究[J]. 植物研究, 2019, 39(5): 683-691.
[2]	林有润. 略论棕榈科与新分出的省藤科的系统分类、演化、区系地理及主要的经济用途[J]. 植物研究, 2002, 22(3): 341-365.
[3]	于晶, 王全喜, 包文美. 中国金粉蕨属孢子形态的研究[J]. 植物研究, 2001, 21(2): 227-230.
[4]	林有润. 中国菊科植物的系统分类与区系的初步研究[J]. 植物研究, 1997, 17(1): 6-27.
[5]	陆兆华, 杨利平, 曹文钟. 中国东北(牛龙)牛儿苗科(Geraniaeeae)花粉形态与系统分类学研究[J]. 植物研究, 1996, 16(3): 315-322.
[6]	于顺利, 林尤兴. 中国产瓦韦属植物的分类学研究[J]. 植物研究, 1996, 16(1): 3-24.
[7]	林有润. 菊科植物的系统分类与区系地理的初步探讨[J]. 植物研究, 1993, 13(2): 151-201.