盐生植物盐节木甜菜碱醛脱氢酶基因的克隆及表达

doi:10.7525/j.issn.1673-5102.2013.03.011

植物研究 ›› 2013, Vol. 33 ›› Issue (3): 317-324.doi: 10.7525/j.issn.1673-5102.2013.03.011

盐生植物盐节木甜菜碱醛脱氢酶基因的克隆及表达

高天鹏^1,4；王春燕²；徐红伟³；孙海丽¹；张鸣⁴；张庆⁴；石晓妮⁴

1.兰州城市学院城市生态与环境生物技术中心，兰州　730070；2.兰州大学学报编辑部，兰州　730000；3.西北民族大学实验中心，兰州　730030；4.兰州城市学院化学与环境科学学院，兰州　730000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-05-20 发布日期:2013-05-20
基金资助:

Molecular Cloning and Expression Analysis of Betaine Aldehyde Dehydrogenase Gene from the Halophyte Halocnermum strobilaceum(Pall.)(Chenopodiaceae)

GAO Tian-Peng;WANG Chun-Yan;XU Hong-Wei;SUN Hai-Li;ZHANG Ming;ZHANG Qing;SHI Xiao-Ni

1.Urban Ecology & Environmental Biotechnology Center,Lanzhou City University,Lanzhou　730000；2.Editorial Department of Journal,Lanzhou University,Lanzhou　730000；3.Science Experimental Center,Northwest University for Nationalities,Lanzhou　730030；4.School Of Chemistry and Environmental Science,Lanzhou City University,Lanzhou　730000

Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
Supported by:

摘要/Abstract

摘要： 采用RT-PCR、RACE技术从盐生植物盐节木(Halocnermum strobilaceum(Pall.) Bieb.)中克隆得到BADH基因的cDNA序列(命名为HsBADH)，3′RACE和 5′RACE测序结果用DNA Man软件拼接获得了1 849 bp全长的HsBADH cDNA序列(GenBank登录号：JN969894)。其开放阅读框为1 503 bp，推测编码501个氨基酸残基，并含有高度保守的十肽基序(VTLELGGKSP)和与酶功能有关的醛脱氢酶Cys残基。其核苷酸序列与几种藜科植物如梭梭、白梭梭、盐穗木、碱蓬、滨藜、菠菜、山菠菜的同源性为86%，与甜土植物水稻的同源性为66%。氨基酸序列与以上两类植物(盐生植物和甜土植物) 的同源性比对分别为89%和70%，说明BADH基因在藜科盐生植物中是一种较高保守的基因。用不同浓度的NaCl胁迫处理盐节木植株，BADH mRNA的表达水平比对照植株高，说明盐节木BADH基因的表达受盐诱导，进一步说明甜菜碱醛脱氢酶催化合成的甜菜碱作为渗透调节的小分子物质，它的积累与盐胁迫存在紧密关联，本研究为进一步从生理和分子水平阐明盐节木的适应盐渍化生境的机制提供一定的参考。

关键词: 盐节木, 甜菜碱醛脱氢酶, 基因克隆, 表达分析

Abstract: According to the published sequences of BADH cDNA of several other plants of Chenopodiaceae, two primers have been designed to amplify the fragments of BADH cDNA from Halocnermum strobilaceum(Pall.) Bieb. through RT-PCR (reverse transcription polymerase chain reaction). A 1 503 bp fragment containing entire betaine aldehyde dehydrogenase (BADH) coding region of 501 amino acids (aa) has been obtained. Nucleotide sequence of HsBADH was similar to the corresponding fragment of BADH cDNA of several other plants, such as Kalidium foliatum, Atriplex centralasiatica, Atriplex hortensis, Spinacia oleracea, Suaeda liaotungensis, Beta vulgaris subsp. Vulgaris, Oryza sativa, etc. Encoded protein by HsBADH and BADH protein from above mentioned plants also shared 89% and 70% identity at the amino acid level. The result showed that BADH gene was conserved, especially in Chenopodiaceae and encoded functional protein may play an important role in high plants during salt stress. Real-time fluorescence quantitative gene expression analysis showed that the level of BADH mRNA in plants treated with different NaCl concentrations was higher than that in the control plants, suggesting that the accumulation of betaine catalyzed by betaine alde-hyde dehydrogenase as an effective osmolyte is important for H.strobilaceum(Pall.) Bieb. during salt stress. The study provided material for further exploring salt tolerant mechanisms of H.strobilaceum(Pall.) Bieb. in physiological and molecular aspects.

Key words: Halocnermum strobilaceum(Pall.) Bieb., betaine aldehydede dehydrogenase, gene cloning, expression analysis

中图分类号:

Q943

高天鹏;王春燕;徐红伟;孙海丽;张鸣;张庆;石晓妮. 盐生植物盐节木甜菜碱醛脱氢酶基因的克隆及表达[J]. 植物研究, 2013, 33(3): 317-324.

GAO Tian-Peng;WANG Chun-Yan;XU Hong-Wei;SUN Hai-Li;ZHANG Ming;ZHANG Qing;SHI Xiao-Ni. Molecular Cloning and Expression Analysis of Betaine Aldehyde Dehydrogenase Gene from the Halophyte Halocnermum strobilaceum(Pall.)(Chenopodiaceae)[J]. Bulletin of Botanical Research, 2013, 33(3): 317-324.

[1]	张昊楠, 陈珊珊, 徐建民, 罗萍, 王晓萍, 许志茹, 范春节. 巨桉EgrWAT1基因克隆和功能初步分析[J]. 植物研究, 2023, 43(4): 601-611.
[2]	宋海云, 张涛, 贺鹏, 郑树芳, 王立丰, 王文林. 澳洲坚果MibZIP1基因克隆及表达规律分析[J]. 植物研究, 2023, 43(1): 131-139.
[3]	黄安瀛, 夏德安, 张洋, 那冬晨, 燕青, 魏志刚. PtrWRKY51基因的克隆及抗旱表达特性分析[J]. 植物研究, 2022, 42(6): 1005-1013.
[4]	陈华峰, 代龙军, 刘明洋, 郭冰冰, 杨洪, 王立丰. 橡胶树胶乳高表达热激蛋白HbHSP90.4基因抗逆功能分析[J]. 植物研究, 2022, 42(6): 1023-1032.
[5]	刘明洋, 肖化兴, 王立丰, 梁晓宇, 张宇, 王萌. 橡胶树热激蛋白HbHSP90.8-1基因的克隆与功能分析[J]. 植物研究, 2022, 42(5): 811-820.
[6]	王宏鹏, 李一丹, 汪耀, 谭晓宇, 陈成彬, 张力鹏. 菊叶薯蓣DcPMK基因克隆及互作蛋白筛选[J]. 植物研究, 2022, 42(5): 855-865.
[7]	杨宇宁, 董昊, 董实伟, 王乃锐, 宋跃, 张含国, 李淑娟. 长白落叶松转录因子LobHLH34克隆及表达分析[J]. 植物研究, 2022, 42(1): 112-120.
[8]	马霜, 王博雅, 曹颖, 胡尚连, 高志民. 毛竹扩展蛋白基因的鉴定及其表达分析[J]. 植物研究, 2022, 42(1): 29-38.
[9]	谭秋锦, 张涛, 韦媛荣, 郑树芳, 汤秀华, 王文林. 澳洲坚果开花相关MiMADS-box基因克隆及表达载体构建[J]. 植物研究, 2021, 41(6): 888-895.
[10]	纪欣童, 于磊, 詹亚光. 水曲柳BZR1基因克隆及其表达模式分析[J]. 植物研究, 2021, 41(5): 744-752.
[11]	柯维忠, 钟雯娟, 刘凯盈, 李祥媛, 尹明华. 黄独微型块茎鲨烯合酶的基因克隆与序列分析[J]. 植物研究, 2021, 41(2): 243-250.
[12]	彭淑萍, 董诚明, 朱畇昊. 响应内生菌侵染的两个地黄茉莉酸合成关键基因的克隆与表达分析[J]. 植物研究, 2021, 41(2): 294-301.
[13]	王肖肖, 覃碧, 杨玉双, 聂秋海, 张继川, 刘实忠. 橡胶草E2泛素结合酶基因TkUBC2基因的克隆及其表达分析[J]. 植物研究, 2021, 41(1): 98-106.
[14]	王文林, 陈海生, 郑树芳, 樊松乐, 王立丰, 谭秋锦, 覃振师, 黄锡云, 贺鹏, 汤秀华, 许鹏. 澳洲坚果MiMYB2基因克隆及结构与功能分析[J]. 植物研究, 2020, 40(6): 913-922.
[15]	王万奇, 齐婉竹, 赵秋爽, 曾栋, 刘轶, 付鹏跃, 曲冠证, 赵曦阳. 白桦BpJMJ18基因启动子克隆及表达分析[J]. 植物研究, 2020, 40(5): 751-759.

盐生植物盐节木甜菜碱醛脱氢酶基因的克隆及表达

Molecular Cloning and Expression Analysis of Betaine Aldehyde Dehydrogenase Gene from the Halophyte Halocnermum strobilaceum(Pall.)(Chenopodiaceae)

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价