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植物研究 ›› 2005, Vol. 25 ›› Issue (3): 373-375.doi: 10.7525/j.issn.1673-5102.2005.03.027

• 论文 • 上一篇    下一篇

高效液相色谱法测定五味子乙素含量

裴毅, 聂江力   

  1. 天津农学院园艺系, 天津 300384
  • 收稿日期:2004-10-18 出版日期:2005-09-15 发布日期:2016-06-14
  • 作者简介:裴毅(1971-),男,讲师,主要从事天然药物化学和药用植物学研究工作。

Determination of Schisandrin B in fruits of Schisandra chinensis with high performance liquid chromagraphy(HPLC)

  1. Tianjin Agricultural college Horticulture, Tianjin 300384
  • Received:2004-10-18 Online:2005-09-15 Published:2016-06-14

摘要: 采用高效液相色谱法,测定了五味子果实中五味子乙素的含量,检测波长为254 nm,标准曲线线性关系R2为0.9907,相对标准偏差为0.48%,平均加样回收率为99.14%。结果表明,此方法准确度较高,为五味子生药及成品药中五味子乙素含量测定提供了一种切实可行的方法。

关键词: 五味子, 高效液相色谱法, 五味子乙素

Abstract: Content of schisandrin B in fruits of Schisandra chinensis was determined with HPLC, the detective wavelength was 254 nm, the R2 of standard curve was 0.990 7 and the relative standard deviation was 0.48% with the average recovery of 99.14%. The result showed that the rate of veracity of this method was high and this provided the determination of schisandrin B of pharmacognostic and finished products a feasible method.

Key words: Schisandra chinensis, HPLC, schisandrin B