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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (4): 466-470.doi: 10.7525/j.issn.1673-5102.2009.04.014

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Screening and Identification of Drought-Sensitive and Insensitive Mutants of Arabidopsis thaliana by Infrared Thermography

SONG Yu-Wei;LI Yu-Ying;YANG Jian-Wei   

  1. (1.College of Life Sciences and Technology,Nanyang Normal University,Nanyang473061) (2.Department of Biology,Henan University,Kaifeng475001)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: SONG Yu-Wei
  • Supported by:
     

Abstract: Drought-sensitive mutant(dri1) and drought-insensitive mutant(drs1) of Arabidopsis thaliana were screened with infrared thermography from an ethyl methane sulfonate-mutagenized population. These mutants were identified as monogenic recessive mutations. Stomata density of these mutants was the same as that of wild type. While, their stomata apertures, water loss and leaf temperature were distinct compared with those of wild type under drought stress. In addition, seed germination test on MS medium indicated that dri1 was more insensitive to ABA, mannitol and NaCl than the wild type, but drs1 was not.

Key words: Arabidopsis thaliana, infrared thermography, drought, mutant

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