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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 200-210.doi: 10.7525/j.issn.1673-5102.2022.02.005

• Molecular biology • Previous Articles     Next Articles

Comparative Transcriptome Analysis of Three Artemisia Species in Qinghai Tibet Plateau

Jingya Yu1,2, Mingze Xia1,2, Hao Xu1,2, Faqi Zhang1()   

  1. 1.Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001
    2.College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049
  • Received:2020-10-08 Online:2022-03-20 Published:2022-02-22
  • Contact: Faqi Zhang E-mail:fqzhang@nwipb.cas.cn
  • About author:Yu Jingya(1995—),female,doctoral student,major in plant systematic and evolution.
  • Supported by:
    Second Tibetan Plateau Scientific Expedition and Research(STEP) Program(2019QZKK0502);Basic Research Project of Qinghai Province(2019-ZJ-7042)

Abstract:

Artemisia dalai-lamaeArtemisia frigida and Artemisia hedinii, the common herbs in arid and semi-arid areas of Northwest China, have the effects of wind prevention and sand fixation, preventing grassland degradation, sterilization and anti-inflammatory, and treating a variety of diseases. The transcriptome of three Artemisia species were sequenced by Illumina method and then 54 268 322, 46 434 864 and 43 971 646 Clean reads were obtained, respectively. SNP and alternative splicing analyses were performed on the sequencing results. Differentially expressed genes were screened from the comparison groups of 6 107(ADL vs AF), 4 822(ADL vs AH) and 3 755(AF vs AH) respectively. Go enrichment annotated the differentially expressed genes into three categories: biological process, cell component and molecular function. KEGG enrichment annotated the differentially expressed genes into 198, 198 and 197 pathways respectively. The top ten high expressed genes in each group were analyzed. We screened out 25 differentially expressed genes related to the active components of alkaloids,terpenoids and flavonoid. This study provid a scientific basis for species identification, stress resistance research and resource utilization of Artemisia.

Key words: Artemisia, comparative transcriptome analysis, SNP, alternative splicing

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