Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs

doi:10.7525/j.issn.1673-5102.2020.01.012

Abstract

Abstract: AtGDPD-Like3 encode glycerophosphodiester phosphodiesterase-like(GDPD) proteins and shv3, its family gene AtGDPD-Like3 mutant, has serious root hair defects. To identify key amino acid sites in AtGDPD-Like3, we constructed S⁵³⁸A, V⁵⁵⁶A and D⁶²⁸A single site mutation of AtGDPD-Like3, which were expressed in atgdpdl3 mutants, respectively. The results showed that AtGDPD-Like3 with V⁵⁵⁶A or D⁶²⁸A mutation could completely restore root hairs growth defects of atgdpdl3, whereas, AtGDPD-Like3 with S⁵³⁸A mutation partially restored root hairs growth defects. These indicate that Ser⁵³⁸ is the key amino acid site in AtGDPD-Like3, which affects function of its protein. Meanwhile, it implies other key amino acid sites in AtGDPD-Like3. The results of this study would lay a foundation for further exploring the functional mechanism of AtGDPD-Like3 protein.

Key words: Arabidopsis thaliana, AtGDPD-Like3, key amino acid locus, point mutation

CLC Number:

Q945.12

WANG Shuang, CHENG Yu-Xiang, XIA De-An. Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs[J]. Bulletin of Botanical Research, 2020, 40(1): 79-84.

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