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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (3): 406-414.doi: 10.7525/j.issn.1673-5102.2018.03.013

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Effects of H2S and PLDα1 on Drought Stress in Arabidopsis thaliana

ZHAO Min, YANG Ning, CHEN Lu, AN Yan-Huang, LI Wen-Ling, ZHAO Feng-Feng   

  1. College of Life Science, Northwest Normal University, Lanzhou 730070
  • Received:2017-12-12 Online:2018-05-15 Published:2018-05-17
  • Supported by:
    National Natural Science Foundation of China(31660116)

Abstract: The experiment was conducted to study the role of phospholipase Dα1(PLDα1) and the gas signal hydrogen sulfide(H2S) in response to drought stress and the possible signal relationship. The wild type Arabidopsis thaliana(WT), PLDα1 mutant(pldα1-1) and the LCD mutant plant(lcd-4) was used as experiment materials, and 0.3 mol·L-1 mannitol was used to simulate drought stress. The content of endogenous H2S, the activities of PLD, L/D-CDes, and the relative expression of PLDα1, LCD and DCD in wild type Arabidopsis were significantly changed under drought stress. Compared with WT, pldα1-1 and lcd-4 were more sensitive to drought condition. Application of NaHS could promote the seed germination and endogenous H2S production in WT, pldα1-1 and lcd-4. Exogenous addition of PA could increase the germination rate and endogenous H2S concentration in WT and pldα1-1, but had no significant effect in lcd-4. The signal molecules H2S and PLDα1 played important roles in the plant response to drought stress, and H2S might be located at downstream of PLDα1 in signal transduction process to regulate the seed germination of A. thaliana.

Key words: Arabidopsis thaliana, drought stress, hydrogen sulfide, phospholipase Dα1

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