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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (4): 559-563.doi: 10.7525/j.issn.1673-5102.2015.04.014

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ISSR Analysis on the Genetic Diversity of Relic Plant Bretschneidera sinensis from Wuyishan Region

LIN Le-Jing1,2;JIN Chu-Qi1;ZHENG Chun-Hao3;ZHOU Ya-Mei1;ZHOU Ying1   

  1. 1.Ningbo City College of Vocational Technology,Ningbo 315100;
    2.Ningbo Key Laboratory of Landscape Plant Development,Ningbo 315502;
    3.Suichang Forestry Bureau fo Zhejiang Province,Suichang 323300
  • Online:2015-07-20 Published:2016-03-09
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Abstract: Bretschneidera sinensis Hemsl., with important research value, is an endangered species endemic in East and South Chin. We used inter-simple sequence repeat(ISSR) markers studied the genetic diversity within and among six populations of B.sinensis. The genetic diversity at species level was high(PPB: 75.70%; HES: 0.304 5; H: 0.450 1). However, relatively low genetic diversity existed within populations. Population in Minjiangyuan(MJY) exhibited the greatest level of variability(PPB: 60.13%; HEP: 0.238 1; H: 0.347 5), whereas the population in Baishanzu(BSZ) showed its own variability at the lowest level(PPB: 44.86%; HE: 0.173 6; H: 0.255 6). By the genetic analysis of the ISSRs, we revealed a relatively high level of genetic differentiation among populations by Nei’s gene diversity statistics(21.81%), Shannon’s information measure(22.79%) and analysis of molecular variance(AMOVA)(18.62%). The main factors responsible for the relatively high level of differentiation among populations were probably related to the predominant outcrossing reproductive system and recent habitat fragmentation, and a significant correlation was between genetic distance and geographic distance(r=0.626 7, P<0.05). The high diversity would hold promise for conservation and restoration efforts to save the endangered species from extinction.

Key words: Bretschneidera sinensis, inter-simple sequence repeats(ISSR), genetic diversity, genetic structure

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