Abstract: Elaeagnus angustifolia L. belonging to Elaeagnaceae is a very important tree species, mainly distributed over the northwest of China and has ability of resistance to saline-alkali. In order to accelerate reproduction and keep its excellent ability, the formation of cotyledon callus and plantlet regeneration from seed explants of Elaeagnus angustifoli were studied. And the organogenesis types of callus and regeneration shoots were discussed. The optimal culture conditions were established with the methods of the orthogonal tests and single factor tests. They are as follows:①explants: the cotyledon of 10 days old seedling;②Efficiency differentiation induction media: MS+0.5 mg·L^-1 NAA+2.0 mg·L^-1 BA+3%sucrose;③Efficiency seedling induction media: MS+0.1 mg·L^-1 NAA+1.0 mg·L^-1 BA+3%sucrose;④Robust seedling media: MS+0.1 mg·L^-1 NAA+1.0 mg·L^-1 BA+0.2 mg·L^-1 GA3+3%sucrose;⑤Rooting media: 1/2MS+1.0 mg·L^-1 ABT1.0 mg·L^-1 +1.5% sucrose.

Key words: Elaeagnus angustifolia, regeneration tissue, differentiation