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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (4): 602-612.doi: 10.7525/j.issn.1673-5102.2022.04.010

• Genetic and Breeding • Previous Articles     Next Articles

Characteristics of Complete Chloroplast Genome and Phylogenetic Analysis of Rhodiola himalensis (Crassulaceae)

Yu ZHANG1,2, Xu SU1,2,3, Yuping LIU1,2(), Tao LIU1,2, Changyuan ZHENG1,2, Dandan SU1,2, Yanan WANG1,2, Ting LÜ1,2   

  1. 1.School of Life Science,Qinghai Normal University,Xining 810008
    2.Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province,Qinghai Normal University,Xining 810008
    2.Academy of Plateau Science and Sustainability,Qinghai Normal University,Xining 810016
  • Received:2021-07-21 Online:2022-07-20 Published:2022-07-15
  • Contact: Yuping LIU E-mail:lyp8527970@126.com
  • About author:ZHANG Yu(1998—),female,postgraduate,mainly engaged in the study of genetic diversity and systematic evolution of alpine plants.
  • Supported by:
    Special Project for Scientific-Technological Basic Resource Investigation(2017FY100202-02);Open Project from Qinghai Research Center of Qilian Mountain National Park;Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibet Plateau in Qinghai Province(2020-ZJ-Y40)


The complete chloroplast genome of Rhodiola himalensis was sequenced, assembled and annotated using high-throughput sequencing technology, and R. himalensis was used as material, an endemic medical species to the Qinghai-Tibet Plateau. The results showed that the complete chloroplast genome of R. himalensis was 151 074 bp in length with a GC content of 37.8%. It had a typical tetrad structure with a long single copy region, a short single copy region and a pair of inverted repeat regions, and the sequence length of them was 82 309, 17 017 and 25 875 bp, respectively. The complete chloroplast genome encoded 130 genes, including 86 protein coding genes, 37 tRNA genes and seven rRNA genes. Based on the chloroplast genome of R. himalensis, a total of 25 513 codons was detected, among which the largest proportion of codons was Leu. In the IRa and IRb regions of R. himalensis, the gene of rps19 and ycf1 was deletion, and the gene of trnH in LSC region was contraction. And R. himalensis and R. sacra had the closest genetic relationship were also found. Besides, the variation frequency of single nucleotide polymorphism(SNP) was the highest within the SSC region. The complete chloroplast genome of R. himalensis was reported, assembled, annotated and analyzed, which could provide the theoretical basis for the study of genetic diversity and rational development and utilization of R. himalensis in the future.

Key words: chloroplast genome, phylogeny, simple sequence repeat, boundary analysis of IR region, Rhodiola himalensis

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