Cloning and Expression Analysis of APETALA2 Gene Promoter from Betula platyphylla

doi:10.7525/j.issn.1673-5102.2018.04.007

Abstract

Abstract: The APETALA2(AP2) transcription factor subfamily is ubiquitous in plants, and involved in the plant growth and development, stress response and signal transduction of various physiological and biochemical reactions. The full-length promoter sequence of AP2 gene was cloned from the genome of Betula platyphylla Suk. By bioinformatics analysis, this sequence has a large number of conserved components such as TATA-box and CAAT box, but also light responsive elements and hormone responsive elements, such as elements that respond to gibberellins, abscisic acid, and methyl jasmonate. The promoter of AP2 gene of B.platyphylla was cloned into pBI121-35S::GUS plant expression vector, named pBI121-proAP2::GUS, Agrobacterium-mediated infection of B.platyphylla and A.thaliana, and by GUS staining analysis, the GUS reporter gene driven by the promoter of AP2 gene is expressed throughout A.thaliana and is expressed in vegetative organs and female flower wings and in flower stalk of B.platyphylla, indicating that it has a priming activity that may participate in the development of this organ.

Key words: Betula platyphylla, AP2, promoter, GUS histochemical staining

CLC Number:

Q943.2

ZHAO Tong, HU Xiao-Qing, ZHU De-Cai, TIAN Jing, XIN Qi-Qi, LIU Xue-Mei. Cloning and Expression Analysis of APETALA2 Gene Promoter from Betula platyphylla[J]. Bulletin of Botanical Research, 2018, 38(4): 535-542.

References

1. Balaji S,Badu M M,Iyer L M,et al. Discovery of the principal specific transcription factors of Apicomplexa and their implication for the evolution of the AP2-integrase DNA binding domains[J]. Nucleic Acids Research,2005,33(13):3994-4006.
2. Riechmann J L,Meyerowitz E M. The AP2/EREBP family of plant transcription factors[J]. Biological Chemistry,1998,379(6):633-646.
3. Guo Y L,Zhu Q L,Zheng S Y,et al. Cloning of a MADS box gene(GhMADS3) from cotton and analysis of its homeotic role in transgenic tobacco[J]. Journal of Genetics and Genomics,2007,34(6):527-535.
4. Maes T,Van De Steene N,Zethof J,et al. Petunia Ap2-like genes and their role in flower and seed development[J]. Plant Cell,2001,13(2):229-244.
5. Nilsson L,Carlsbecker A,Sundås-Larsson A,et al. APETALA2 like genes from Picea abies show functional similarities to their Arabidopsis homologues[J]. Planta,2007,225(3):589-602.
6. Guillaumot D,Lelu-Walter M A,Germot A,et al. Expression patterns of LmAP2L1 and LmAP2L2 encoding two-APETALA2 domain proteins during somatic embryogenesis and germination of hybrid larch(Larix×marschlinsii)[J]. Journal of Plant Physiology,2008,165(9):1003-1010.
7. 周盛梅,宿红艳,王磊,等. 苹果apetala2同源基因的克隆和转化研究[J]. 园艺学报,2006,33(2):239-243.Zhou S M,Su H Y,Wang L,et al. Cloning and transformation of apetala2 homologues genes from apple[J]. Acta Horticulturae Sinica,2006,33(2):239-243.
8. Zhuang J,Deng D X,Yao Q H,et al. Discovery,phylogeny and expression patterns of AP2-like genes in maize[J]. Plant Growth Regulation,2010,62(1):51-58.
9. Zhao L F,Xu S B,Chai T Y,et al. OsAP2-1,an Ap2-like gene from Oryza sativa,is required for flower development and male fertility[J]. Sexual Plant Reproduction,2006,19(4):197-206.
10. 刘雪梅,杨传平. 东北地区白桦雌配子体的形成与胚胎发育研究[J]. 植物学报,2005,22(2):147-152.Liu X M,Yang C P. Megagametophyte and embryo development of Betula platyphylla in northeastern China[J]. Chinese Bulletin of Botany,2005,22(2):147-152.
11. 刘雪梅,杨传平,耿峰. 白桦胚珠发育及胚、胚乳发育关系的研究[J]. 植物研究,2005,25(3):322-326.Liu X M,Yang C P,Geng F. Development of ovule and relation between embryo and endosperm developments of Betula platyphylla[J]. Bulletin of Botanical Research,2005,25(3):322-326.
12. 刘雪梅,杨传平. 白桦雌雄花发育周期的时序特征[J]. 林业科学,2006,42(12):28-32.Liu X M,Yang C P. Temporal characteristics of developmental cycles of female and male flowers in Betula platyphylla in northeastern China[J]. Scientia Silvae Sinicae,2006,42(12):28-32.
13. 姜静,李同华,庄振东,等. 白桦雌花发育、大孢子发生及胚胎发育的解剖学观察[J]. 植物研究,2003,23(1):46-50.Jiang J,Li T H,Zhuang Z D,et al. The anatomic observation of the development of female flower Megasporegensis and the development of embryo of Betula platyhylla[J]. Bulletin of Botanical Research,2003,23(1):46-50.
14. 李萌,哈努拉·塔斯肯,陈卓,等. 白桦4CL基因启动子克隆及表达分析[J]. 中国农学通报,2017,33(5):29-34.Li M,Tasiken H,Chen Z,et al. Cloning and expression analysis of 4CL gene promoter of Betula platyphylla[J]. Chinese Agricultural Science Bulletin,2017,33(5):29-34.
15. 郭勇,王玉成,王智博. 一种基于农杆菌介导的拟南芥瞬时转化技术优化[J]. 东北林业大学学报,2016,44(6):41-44,83.Guo Y,Wang Y C,Wang Z B. Optimizing transient genetic transformation method on Arabidopsis plants mediated by Agrobacterium tumefaciens[J]. Journal of Northeast Forestry University,2016,44(6):41-44,83.
16. Park W,Li J J,Song R T,et al. CARPEL FACTORY,a Dicer homolog,and HEN1,a novel protein,act in microRNA metabolism in Arabidopsis thaliana[J]. Current Biology,2002,12(17):1484-1495.
17. Peragine A,Yoshikawa M,Wu G,et al. SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis[J]. Genes & Development,2004,18(19):2368-2379.
18. Yant L,Mathieu J,Dinh T T,et al. Orchestration of the floral transition and floral development in Arabidopsis by the bifunctional transcription factor APETALA2[J]. Plant Cell,2010,22(7):2156-2170.
19. Teotia S,Tang G. To bloom or not to bloom:role of microRNAs in plant flowering[J]. Molecular plant,2015,8(3):359-377.
20. Aukerman M J,Sakai H. Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes[J]. Plant Cell,2003,15(11):2730-2741.
21. Jung J H,Seo Y H,Seo P J,et al. The GIGANTEA-regulated microRNA172 mediates photoperiodic flowering independent of CONSTANS in Arabidopsis[J]. Plant Cell,2007,19(9):2736-2748.
22. Chen X M. A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development[J]. Science,2004,303(5666):2022-2025.
23. Jung J H,Seo P J,Kang S K,et al. MiR172 signals are incorporated into the miR156 signaling pathway at the SPL3/4/5 genes in Arabidopsis developmental transitions[J]. Plant Molecular Biology,2011,76(1-2):35-45.
24. Ohto M,Floyd S K,Fischer R L,et al. Effects of APETALA2 on embryo,endosperm,and seed coat development determine seed size in Arabidopsis[J]. Sexual Plant Reproduction,2009,22(4):277-289.
25. 张妍,刘瀛,孙丰宾,等. 白桦APETALA2(AP2)转录因子基因的分离及其表达[J]. 林业科学研究,2012,25(2):254-260.Zhang Y,Liu Y,Sun F B,et al. Isolation and expression of APETALA2 transcription factor gene in Betula platyphylla[J]. Forest Research,2012,25(2):254-260.
26. Chung B Y,Simons C,Firth A E,et al. Effect of 5'UTR introns on gene expression in Arabidopsis thaliana[J]. BMC Genomics,2006,7:120.
27. Kim M J,Kim H,Shin J S,et al. Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5'-UTR intron[J]. Molecular Genetics and Genomics,2006,276(4):351-368.
28. Samadder P,Sivamani E,Lu J L,et al. Transcriptional and post-transcriptional enhancement of gene expression by the 5'UTR intron of rice rubi3 gene in transgenic rice cells[J]. Molecular Genetics and Genomics,2008,279(4):429-439.
29. Karthikeyan A S,Ballachanda D N,Raghothama K G. Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis[J]. Physiologia Plantarum,2009,136(1):10-18.

[1]	Binghua CHEN, Jie ZHANG, Guifeng LIU, Siting LI, Yuanke GAO, Huiyu LI, Tianfang LI. Selection of Excellent Families and Evaluation of Selection Method for Pulpwood Half-sibling Families of Betula platyphylla [J]. Bulletin of Botanical Research, 2023, 43(5): 690-699.
[2]	Jingzhe WANG, Chaokui NIU, Xinyuan LIANG, Chenjing SHEN, Jing YIN. Regulation of Salicylic Acid on Tolerance to Saline Alkali Stress at Seedling Stages of Betula platyphylla [J]. Bulletin of Botanical Research, 2023, 43(3): 379-387.
[3]	Jinxia DU, Tingting SHEN, Haoran WANG, Yiping LIN, Huiyu LI, Lianfei ZHANG. Construction of Suppression Expression Vector and Genetic Transformation of BpSPL9 gene from Betula platyphylla [J]. Bulletin of Botanical Research, 2023, 43(1): 30-35.
[4]	Kun CHEN, Gonggui FANG, Huaizhi MU, Jing JIANG. Analysis of the Promoter Sequence and Response Characteristics of the BpPIN3 gene in Betula platyphylla [J]. Bulletin of Botanical Research, 2022, 42(4): 592-601.
[5]	Wang Xie, Tianjing Li, Xinyao Li, Fenming Yang, Yin’an Yan, Yongfeng Gao. Identification of PeNAC121 Gene Promoter and Stress Response Pattern Analysis in Populus euphratica [J]. Bulletin of Botanical Research, 2022, 42(2): 234-242.
[6]	Yunli Yang, Chang Qu, Yang Wang, Guifeng Liu, Jing Jiang. Tissue-specific Expression and Analysis of Exogenous Hormone Response of BpPIN5 Gene Promoter in Betula platyphylla [J]. Bulletin of Botanical Research, 2022, 42(1): 104-111.
[7]	Qing MA, Fang-Rui LI, Gui-Feng LIU, Hui-Yu LI. Analysis on the Growth Characters of Betula platyphylla in the Aerospace Mutation [J]. Bulletin of Botanical Research, 2021, 41(4): 540-546.
[8]	Yi-Ping GUO, Jia-Xin LIU, Ying YU, Chao WANG, Chuan-Ping YANG. Expression Profile Analysis ofXylem Development Regulated by BpNAC012 Gene from Betula platyphylla [J]. Bulletin of Botanical Research, 2021, 41(2): 251-261.
[9]	LIU Jia-Xin, LIU Hui-Zi, SHI Jing-Jing, YU Ying, WANG Chao. Expression of MYB Genes of Birch in Response to Hormones,Salt and Drought [J]. Bulletin of Botanical Research, 2020, 40(5): 743-750.
[10]	WANG Wan-Qi, QI Wan-Zhu, ZHAO Qiu-Shuang, ZENG Dong, LIU Yi, FU Peng-Yue, QU Guan-Zheng, ZHAO Xi-Yang. Cloning and Expression Analysis of BpJMJ18 Gene Promoter in Betula platyphylla [J]. Bulletin of Botanical Research, 2020, 40(5): 751-759.
[11]	JIANG Cheng, ZHANG Xi, TIAN Qing, LI Li. Isolation of the BpbHLH112 Gene and Expression Analysis of Its Promoter in Betula platyphylla [J]. Bulletin of Botanical Research, 2020, 40(4): 583-592.
[12]	SUN Shuo, WANG Xiu-Wei, DU Meng-Tian, LI Jing-Hang, WANG Bo-Yi, LIU Gui-Feng. Root CO₂ Efflux Variations of Betula platyphylla Among Sites and Root Diameter Classes in 12 Different Provenances [J]. Bulletin of Botanical Research, 2020, 40(3): 476-480.
[13]	ZHANG Jiao, WANG Xuan, ZHANG Liang-Bo, LIU Zhi-Xiong. Expression Analysis of the FaesAP2B in Mutant Buckwheat with Long Pistil and Long Stamen [J]. Bulletin of Botanical Research, 2020, 40(2): 266-273.
[14]	QIN Lin-Lin, ZHANG Xi, JIANG Cheng, LI Li. Cloning and Functional Analysis of BpZFP4 Promoter from Birch(Betula platyphylla) [J]. Bulletin of Botanical Research, 2019, 39(6): 917-926.
[15]	FAN Er-Qin, LIU Cai-Xia, FU Peng-Yue, YANG Chuan-Ping, QU Guan-Zheng. Expression Pattern of PagC3H3 Gene in Populus alba×P.glandulosa [J]. Bulletin of Botanical Research, 2019, 39(4): 521-528.