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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (3): 323-328.doi: 10.7525/j.issn.1673-5102.2006.03.015

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Tissue culture of Dioscorea opposita “foshou”

LIU Jin-Ying;XU You-Ming;LI Shuang-Lai;GUO Chun-Feng;GUO Zhi-Cheng   

  1. 1.Huazhong Agricultural University,Wuhan 430070 2.Hubei Agricultural Institute,Wuhan 430064 3.Agricultural bureau of Wuxue City,Wuxue 435400
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-20 Published:2006-05-20
  • Contact: LIU Jin-Ying
  • Supported by:

Abstract: Tissue culture of Dioscorea opposita “foshou” was studied with explants such as tubers,leaves,stem segments. The results showed that the media of MS+6-BA1.0 mg·L-1+NAA0.1 mg·L-1 was for tubers and the media of MS+6-BA0.5~1.0 mg·L-1+NAA2.0 mg·L-1 was for leaves in the dark condition. The tissue culture of stems was conducted in light condition. The optimum media was MS + 6-BA1.0 mg·L-1 + NAA0.5 mg·L-1 for the stem without the node. MS+ 6-BA0.5~1.0 mg·L-1 + NAA0.1 mg·L-1 was suitable for primary culture of the stem with the node, the optimum propagating media was MS+6-BA0.5 mg·L-1+ NAA0.1 mg·L-1, roots were induced on the media of 1/2MS+NAA0.5 mg·L-1.

Key words: Dioscorea opposita &ldquo, foshou&rdquo, tubers, leaves, stem segments, tissue culture

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