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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (4): 626-636.doi: 10.7525/j.issn.1673-5102.2022.04.012

• Molecular biology • Previous Articles     Next Articles

Evolutionary Analysis of Chloroplast Genome of Parnassia

Mingze XIA1,2, Faqi ZHANG1, Xiaofeng CHI1, Shuang HAN1,2, Shilong CHEN1()   

  1. 1.Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008
    2.University of Chinese Academy of Sciences,Beijing 100039
  • Received:2021-11-03 Online:2022-07-20 Published:2022-07-15
  • Contact: Shilong CHEN E-mail:slchen@nwipb.cas.cn
  • About author:XIA Mingze(1994—),male,Ph.D,major in taxonomy phylogeny and evolution of plants.
  • Supported by:
    The Second Tibetan Plateau Scientific Expedition and Research(STEP) program(2019QZKK05020102)


Sequence variation and gene composition of chloroplast genome could effectively reflect the phylogenetic and evolutionary relationships among plants. In this study, DNA libraries were prepared and sequenced by the Illumina HiSeq, chloroplast genomes offive species of Parnassia and allied genera were assembled respectively, and the structural characteristics, sequence genetic variation and codon preference of protein coding genes of Parnassia chloroplast genome with related species were compared and analyzed. The results showed that the chloroplast genome of Parnassia was conserved tetrad structure, and pseudogenization of several genes were found in P. palustris, but the chloroplast gene composition of other species was the same, encoding 115 genes. Compared with the relative species, the intron of rpl16 gene were lost in all species of Parnassia. The ratio of nonsynonymous/synonymous substitution rate of protein coding genes was low, and chloroplast genes might experience purification selection. Codon preference was consistent with the phylogenetic relationship shown by protein coding sequences. This study reveals that selection pressure may play an important role in the evolution of protein-coding genes in the chloroplast genome of Parnassia, which could enhance the understanding about evolution and adaptation of this genus.

Key words: Parnassia, plastome, codon usage bias, phylogeny, evolution

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