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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (1): 117-124.doi: 10.7525/j.issn.1673-5102.2018.01.014

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Characteristic of Molecular Evolution for Lilium pumilum Redouté on Southeast of the Qinghai-Tibetan Plateau using nrDNA ITS and cpDNA petB/petD Sequence Analysis

JIANG Fu-Juan1,3, TANG Nan1,2, TANG Dao-Cheng1, JU Xiu-Ting1, SUN Jing-Qing4   

  1. 1. Plateau Flower Research Center of Qinghai University/The key laboratory of landscape plants of Qinghai Province, Xining 810016;
    2. State Key Laboratory Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, Xining 810016;
    3. Datong County Forestry Bureau, Datong 810100;
    4. Xining People's Park, Xining 810008
  • Received:2017-05-24 Online:2018-01-15 Published:2018-01-06
  • Supported by:
    Natural Science Foundation of China 31660582(Genetic mapping of resistance gene for tulip breaking virus);“Thousand Talents Program”of Qinghai Province;CAS“Light of West China”Program

Abstract: In this study, total DNA was extracted from Lilium pumilum on southeast of the Qinghai-Tibetan Plateau using modified CTAB method. Using genome DNA as template, nrDNA ITS and cpDNA petB/petD regions were amplified, purified and sequenced. By sequence alignment, it was known that length of nrDNA ITS sequence was 696 bp, of which 4 variable sites with a percentage of 0.72% were found., The G+C content of the ITS sequence was 60.4% and 7 haplotypes were produced. The length of cpDNA petB/petD sequence was 616 bp, of which 1 was variable sites with a percentage of 0.16% was found. The G+C content of the sequence was 34.6% and 2 haplotypes were found. The petB/petD region of L.pumilum was more conserved than ITS sequences and evolved more slowly. The present distribution range of L.pumilum has probably experienced range expansion by the haplotype analysis of mismatch distribution and neutral test, and AMOVA analysis showed that the genetic variation of L.pumilum mainly existed within populations. The NST >GST(P>0.01)further showed that there was no significant phylogeographic structure of genetic variation in L.pumilum. Therefore, the nrDNA ITS sequence of L.pumilum was more suitable for phylogeographic study of this species.

Key words: southeastern of Qinghai-Tibetan Plateau, Lilium pumilum, ITS, petB/petD, molecular evolution

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