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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 412-423.doi: 10.7525/j.issn.1673-5102.2022.03.011

• Molecular biology • Previous Articles     Next Articles

Genome-wide Identification and Expression Analysis of nsLTP Gene Family in Populus trichocarpa

He CHENG1, Shuanghui TIAN1, Yang ZHANG1, Cong LIU1, De’an XIA1, Zhigang WEI2()   

  1. 1.State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
    2.Saline Soil Research Center,National Forestry and Grassland Administration,Chinese Academy of Forestry,Beijing 100091
  • Received:2021-03-28 Online:2022-05-20 Published:2022-05-20
  • Contact: Zhigang WEI E-mail:zhigangwei1973@163.com
  • About author:CHENG He(1996—),female,master,main research direction:tree resistance breeding.
  • Supported by:
    the National Natural Science Foundation of China(31770640);Subproject of the National Key Research and Development Program(2017YFC0504102-04)


Non-specific lipid transfer proteins(nsLTP), a polygene family of basic lipid transfer proteins, are responsible for the transfer of phospholipids between fatty acids and membrane in vitro, and play an important role in plant growth and development and the response to stress. There have been no reports on the nsLTP family of Populus trichocarpa. In this study, the number of genes, genetic relationship, gene structure and conserved motif of coding proteins of PtrnSLTP family members were analyzed at the genome-wide level. The results showed that the PtrnsLTP family was composed of 39 genes and evolved into five subfamilies, among which six genes in subfamily A, two genes in subfamily B, 13 genes in subfamily C, three genes in subfamily D, and 15 genes in subfamily E. PtrnsLTP family contained seven pairs of paragenetic homologous genes, among which one pair was greater than one, and the six pairs of Ka/Ks were all far less than one. Moreover, these six pairs of genes were all on the same large evolutionary branch, and different evolutionary pressures led to functional differentiation among genes. Both encoded proteins contained conserved motifs in Motif 1 and Motif 2. Using qRT-PCR technology combined with P. trichocarpa data to study the tissue expression of PtrnsLTP and the response characteristics of salt stress, it was found that all family members expressed in the roots, stems and leaves of P. trichocarpa. The results were basically consistent with the prediction results by the bioinformatics analysis of the website. There were 11, 15 and 13 members respectively having higher expression in roots, stems and leaves, indicated that this gene family was involved in the growth and development of different tissues of P. trichocarpa. Under NaCl stress, among the 39 genes in the family, 26 genes expressed in the root and 14 genes expressed in the leaves increased with the increase of stress time, while 32 genes expressed in the stem first increased and then decreased. The results have a positive role in promoting the identification of the biological function of PtrnsLTP family genes and the work of salt stress response gene resources.

Key words: Populus trichocarpa, nsLTP, bioinformatics analysis, salt stress

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