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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (1): 98-106.doi: 10.7525/j.issn.1673-5102.2021.01.013

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Cloning and Expression Analysis of E2 Ubiquitin-conjugating Enzyme Gene TkUBC2 in Taraxacum kok-saghyz Rodin

Xiao-Xiao WANG1,2, Bi QIN2, Yu-Shuang YANG2, Qiu-Hai NIE3, Ji-Chuan ZHANG4, Shi-Zhong LIU2()   

  1. 1.College of Tropical Crops,Hainan University,Haikou 570228
    2.Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
    3.Beijing Linglong Dandelion Technology and Development Ltd,Beijing 110000
    4.College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029
  • Received:2020-07-02 Online:2021-01-20 Published:2021-01-05
  • Contact: Shi-Zhong LIU E-mail:sz_liu@126.com
  • About author:WANG Xiao-Xiao(1996—),female,master,mainly engaged in the agronomy and seed industry.
  • Supported by:
    National Key Research and Development Project(2017YFB0306901);Research and Development Project of Beijing Linglong Dandelion Technology and Development Ltd.(Germplasm collection, innovation and breeding of Taraxacum kok-saghyz Rodin);Earmarked Funds from Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences(1630022019015)


In order to analyze the function of E2 ubiquitin-conjugating enzyme(UBC) in stress response and signal transduction, an UBC gene was cloned from Taraxacum kok-saghyz Rodin(TKS) line 1151 and named as TkUBC2. The ORF of this gene was 459 bp and encoded 152 aa. Sequence alignment analysis showed that UBC2 shared high level homology among different species, and TkUBC2 had more than 99% similarity with Lactuca sativa LsUBC2 and Helianthus annuus HaUBC2. qRT-PCR was used to analyze the expression pattern of TkUBC2in TKS. The results indicated that TkUBC2 was ubiquitously expressed in different tissues. Moreover, TkUBC2expression was down-regulated by treatments of PEG6000 simulated drought stress, mannitol mediated osmotic stress and plant hormone i.e. methyl jasmonate(MeJA), abscisic acid(ABA) and ethylene(ET) respectively, but its expression was up-regulated by the treatments of NaCl salt stress and UV radiation respectively. The results of this study suggested that TkUBC2 was involved in response to various stress conditions, hormone signaling and DNA damage repair process, which lay a good foundation for further revealing the function of TkUBC2 in TKS.

Key words: Taraxacum kok-saghyz Rodin, TkUBC2, abiotic stress, expression analysis

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