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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (3): 441-446.doi: 10.7525/j.issn.1673-5102.2020.03.016

• Research Report • Previous Articles     Next Articles

Expression and Function of BoNR8 lncRNA in Cabbage under Cold Stress

YANG He1, ZHANG Nan1, LIU Zi-Guang2, LIN Jian-Hui1, SUN Shi-Chen3, LIU Sheng-Yi4, CEN Xi1, WU Juan1   

  1. 1. Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field of Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040;
    2. Institute of Animal Science, Heilongjiang Academy of Agricultural Sciences, Harbin 150028;
    3. Institute of Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150028;
    4. Mudanjiang Medical University, Mudanjiang 157011
  • Received:2019-10-17 Online:2020-05-05 Published:2020-05-29
  • Supported by:
    Heilongjiang Provincial Science Fund Project(LH2019C007);Fundamental Research Funds for Central Universities(2572019CT03);Key Laboratory of Salt and Alkali Vegetation Restoration and Reconstruction of the Northeast Oilfield(SAVER1702)

Abstract: Long non-coding RNA(lncRNA) with longer than 200 bp in length do not contain open reading frames, and they generally do not have a protein-coding function. They are ubiquitous in the body and play a variety of biological roles. There are few studies on lncRNA specifically expressed in the germination stage of plant seeds. BoNR8 lncRNA is a long non-coding RNA(about 272 bp) transcribed by RNA polymerase Ⅲ in cabbage. Previous studies have found that it is specifically expressed in the seed germination stage, and the overexpression of BoNR8 lncRNA in Arabidopsis inhibits seed germination under normal conditions and reduces the sensitivity of germinated seeds to ABA. In this study, we analyzed the sequence of BoNR8 lncRNA and found that there was a cold stress motif(ATATAAATAAAT) in the transcribed region, and this motif exists in the largest stem loop of the secondary structure of BoNR8 lncRNA. Biochemical experiments further showed that BoNR8 lncRNA responded to low temperature environment, and its overexpression inhibited seed germination of Arabidopsis thaliana under low temperature stress. The discovery of low-temperature related functions of BoNR8 lncRNA provides new materials for low temperature resistance research of plant and enriches the study of low temperature resistance mechanism of plant.

Key words: long non coding RNA, Brassica oleracea L., cold stress, seed germination

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