Expression and Function of BoNR8 lncRNA in Cabbage under Cold Stress

doi:10.7525/j.issn.1673-5102.2020.03.016

Abstract

Abstract: Long non-coding RNA(lncRNA) with longer than 200 bp in length do not contain open reading frames, and they generally do not have a protein-coding function. They are ubiquitous in the body and play a variety of biological roles. There are few studies on lncRNA specifically expressed in the germination stage of plant seeds. BoNR8 lncRNA is a long non-coding RNA(about 272 bp) transcribed by RNA polymerase Ⅲ in cabbage. Previous studies have found that it is specifically expressed in the seed germination stage, and the overexpression of BoNR8 lncRNA in Arabidopsis inhibits seed germination under normal conditions and reduces the sensitivity of germinated seeds to ABA. In this study, we analyzed the sequence of BoNR8 lncRNA and found that there was a cold stress motif(ATATAAATAAAT) in the transcribed region, and this motif exists in the largest stem loop of the secondary structure of BoNR8 lncRNA. Biochemical experiments further showed that BoNR8 lncRNA responded to low temperature environment, and its overexpression inhibited seed germination of Arabidopsis thaliana under low temperature stress. The discovery of low-temperature related functions of BoNR8 lncRNA provides new materials for low temperature resistance research of plant and enriches the study of low temperature resistance mechanism of plant.

Key words: long non coding RNA, Brassica oleracea L., cold stress, seed germination

CLC Number:

S635.1

YANG He, ZHANG Nan, LIU Zi-Guang, LIN Jian-Hui, SUN Shi-Chen, LIU Sheng-Yi, CEN Xi, WU Juan. Expression and Function of BoNR8 lncRNA in Cabbage under Cold Stress[J]. Bulletin of Botanical Research, 2020, 40(3): 441-446.

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