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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (6): 921-930.doi: 10.7525/j.issn.1673-5102.2018.06.016

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Cloning and Expression Analysis of Glutelin GluC Gene Promoter in Rice

LI Wen-Jing1, SUN Yan-Xiang1,2   

  1. 1. College of Life Science, Langfang Teachers University, Langfang 065000;
    2. Genetic and Breeding Research Insitute, College of Life Science, Langfang Teachers University, Langfang 065000
  • Received:2018-06-19 Online:2018-11-15 Published:2018-11-01
  • Supported by:
    National Natural Science Foundation of China(31500196);Key development project of genetics in Hebei Province(201221)

Abstract: Rice glutelin, which is expressed in endosperm exclusively, is ideal candidate for isolating promoters with endosperm specificity. We isolated the GluC 5'-flanking sequence pGluC. Analysis of pGluC sequence by PlantCARE and PLACE revealed it contained a series of endosperm specific elements, such as Skn-1 motif and ACGT-box. Thus, we constructed the pGluC and truncated pGluC into pGPTV-GUS vector and transferred the vectors into rice. We performed histochemical assay and GUS activity detection. The full-length and -1 911,-1 611, -1 311 bp promoters were mainly accumulated in endosperm. The -999, -451, -203, -102 bp promoters were mainly accumulated in endosperm, and less strongly in roots, stems and leaves, which changed their endosperm-specific expression pattern. Our findings would contribute to the selection of a suitable promoter for crop molecular improvement.

Key words: rice, promoter, cis-acting element, GUS histochemical staining, quantitative GUS assay

CLC Number: