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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (6): 855-860.doi: 10.7525/j.issn.1673-5102.2017.06.008

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Effect of Ca2+ on Seed Germination and Seedling Formation of Oat Induced by H2O2 under Saline-Alkali Complex Stress

LIU Jian-Xin1,2, LIU Xiu-Li1,2, WANG Jin-Cheng1,2, JIA Hai-Yan1,2   

  1. 1. Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Qingyang 745000;
    2. College of Life Science and Technology, Longdong University, Qingyang 745000
  • Received:2017-03-03 Online:2017-11-15 Published:2017-11-25
  • Supported by:
    The project was financially supported by the science and technology plan project of Qingyang city in Gansu province(KZ2014-19)

Abstract: Salinity-alkalinity stress is an important factor of restricting crop high-yield and good quality, Ca2+ and H2O2 as signal molecules involved in crop response to stress. In order to understand whether Ca2+ is involved in the regulation of H2O2 on the seed germination and seedling formation in plant under saline-alkali stress, using subordinate function analysis method, we studied the effects of extracellular dissociation Ca2+ chelant EGTA, plasma membrane Ca2+ channel blockers LaCl3, tonoplast Ca2+ release inhibitor ruthenium red(RR) and H2O2 on seed germination and seedling formation under saline-alkali complex stress(NaCl:Na2SO4:NaHCO3:Na2CO3=12:8:9:1) with oat(Avena nude). The results showed that 25-200 mmol·L-1 saline-alkali complex stress significantly inhibited seed germination and seedling formation of oat, and its inhibition was more obvious as the concentration was increased. While 0.001-2 mmol·L-1 H2O2 could promote the seed germination and seedling formation of oat, and 0.5 mmol·L-1 H2O2 significantly relieve the inhibition of 75 mmol·L-1 saline-alkali complex stress to seed germination and seedling formation of oat. After EGAT treatment, combining with LaCl3 and RR, the effect of H2O2 was decreased. Therefore, Ca2+ was involved in the signal transduction of seed germination and seedling formation of oat induced by H2O2 under saline-alkali mixed stress.

Key words: Ca2+, H2O2, saline-alkali mixed stress, oat, seed germination and seedling formation

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