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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (1): 68-76.doi: 10.7525/j.issn.1673-5102.2015.01.012

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SSR of Genetic Distance and Clustering Analysis on Different Populus Section Clones

    

  1. 1.Tree Seedling Management Station,The Forestry Department of Jilin Province,Changchun 130000;
    2.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040;
    3.Beijing Municipal Gardens Bureau,Beijing 100029;
    4.The Management of Student Activity Center in Beijing,Beijing 100061;
    5.Forest Seed Orchard of Siping,Siping 136000;
    6.Agricultural Bureau of Taobei District,Baicheng 137000
  • Online:2015-01-15 Published:2015-03-11
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Abstract: Simple Sequence Repeat (SSR) was employed to study the genetic analysis and genetic distance (GD) among 48 clones in Populus L. Sixteen polymorphism markers produced 81 alleles (size ranged from 100-600 bp) totally. GD ranged from 0.061 7-0.530 9 among 48 poplar clones with the average of 0.250 4. GD ranged from 0.148 2-0.274 1 between inter-section and from 0.330 2-0.401 2 between different sections. By cluster analysis, 48 clones were clustered into 5 groups at 0.70, of which the clones divided into 5 section (sect.Leuce; sect.Aigeiros; sect.Tacamahaca; sect.Leucoides and sect.Turanga) clearly. Genetic distance of different section of Populus by molecular method can provide theoretical basis for cross breeding and molecular breeding in poplar.

Key words: Populus spp.,Simple Sequence Repeat(SSR), genetic distance(GD), cluster analysis

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