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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (1): 47-51.doi: 10.7525/j.issn.1673-5102.2015.01.009

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Cloning and SNP Analysis of DREB2 Gene in Fourteen Leymus Species


  1. College of Life Science,Sichuan Agricultural University,Ya’an 625014
  • Online:2015-01-15 Published:2015-03-11
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Abstract: DREB2 transcription factor plays an important role in response to abiotic stresses of drought and salinity. We obtained the DREB2 sequences from 14 Leymus species. By sequence analysis, the DREB2 gene and amino acid are conserved among 14 Leymus species. The similarity of nucleotide sequence and amino acid sequence are 98.05% and 97.78%, respecctively. There are 3 bp deletion CTT in L.mollis. Among these sequences, there are 79 SNP identified. The polymorphism frequence is 1SNP/10.6 bp. Fourteen Leymus species can be well separated by the phylogenetic trees of DREB2 gene.

Key words: Leymus, DREB2, single nucleotide polymorphisms(SNP), sequences analysis

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