Cloning and SNP Analysis of DREB2 Gene in Fourteen Leymus Species

doi:10.7525/j.issn.1673-5102.2015.01.009

Abstract

Abstract: DREB2 transcription factor plays an important role in response to abiotic stresses of drought and salinity. We obtained the DREB2 sequences from 14 Leymus species. By sequence analysis, the DREB2 gene and amino acid are conserved among 14 Leymus species. The similarity of nucleotide sequence and amino acid sequence are 98.05% and 97.78%, respecctively. There are 3 bp deletion CTT in L.mollis. Among these sequences, there are 79 SNP identified. The polymorphism frequence is 1SNP/10.6 bp. Fourteen Leymus species can be well separated by the phylogenetic trees of DREB2 gene.

Key words: Leymus, DREB2, single nucleotide polymorphisms(SNP), sequences analysis

CLC Number:

GAO Gang；DENG Jia-Bin；GOU Xue-Mei；WANG Qian；ZHANG Yan；YANG Rui-Wu*. Cloning and SNP Analysis of DREB2 Gene in Fourteen Leymus Species[J]. Bulletin of Botanical Research, 2015, 35(1): 47-51.

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