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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (6): 685-688.doi: 10.7525/j.issn.1673-5102.2012.06.009

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Tissue Culture and Plant Regeneration of Rumex gmelini Turcz.

WU Bai-Yan;XIE Yan-Mei;WANG Zhen-Yue*   

  1. 1.College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040;2.Harbi Pharm. Group Sanchine Pharmaceutical Holding Co,Ltd,Harbin 150069
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Contact: WANG Zhen-Yue
  • Supported by:

Abstract: With the hypocotyls and seedling rhizomes of Rumex gmelini Turcz. as explants, the tissue culture of R.gmelini was studied by adding different concentrations of 6-BA and 2,4-D into MS basic mediums. The results showed that the better materials for multiple shoot clumps induction were seedling rhizomes; the best medium for multiple shoot clumps induction was MS+6-BA 3.0 mg·L-1+2,4-D 0.1 mg·L-1, the induction rate was 81.2%; the rooting medium was MS basic mediums without hormones, the rooting rate could get 92%, and under this conditions the plant regeneration could be obtained rapidly. After transplantation, about 80% of these seedlings could survive.

Key words: Rumex gmelini Turcz, tissue culture, plant regeneration

CLC Number: