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植物研究 ›› 2014, Vol. 34 ›› Issue (3): 380-385.doi: 10.7525/j.issn.1673-5102.2014.03.015

• 论文 • 上一篇    下一篇

山新杨组培苗生根移栽方法

王娜1;窦恺2;王志英2;张荣沭3;王玉成1;刘志华2*   

  1. 1.东北林业大学林木遗传育种国家重点实验室,哈尔滨 150040;2.东北林业大学林学院,哈尔滨 150040;3.东北林业大学园林学院,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-05-20 发布日期:2014-05-20
  • 通讯作者: 刘志华
  • 基金资助:
     

Rooting and Transplanting Method of Populus davidiana×P.bolleana Tissue Culture Seedlings

WANG Na;DOU Kai;WANG Zhi-Ying;ZHANG Rong-Shu;WANG Yu-Cheng;LIU Zhi-Hua*   

  1. 1.State Key Laboratory of Tree Genetics Breeding,Northeast Forestry University,Harbin 150040;2.School of Forestry,Northeast Forestry University,Harbin 150040;3.College of Landscape,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: LIU Zhi-Hua
  • Supported by:
     

摘要: 从炼苗,保湿,防污染等方面,研究出一种新型高效的山新杨组培苗生根移栽方法。首先,获取山新杨组培苗,将其用液体生根培养基在大试管中生根12~15 d;再放入无菌自来水中水培炼苗2 d;之后将幼苗移栽入土,上扣透明塑料水杯保湿,15 d后移去水杯;带土移栽到大田中。此法可使组培苗移栽成活率提高至98%以上,且本法经济简便,规模小,效率高,也适用于其它木本植株,便于推广。

关键词: 山新杨, 组织培养, 生根, 移栽, 成活率

Abstract: A new typical and high efficient rooting and transplanting method of Populus davidiana×P.bolleana was set up from hardening off seedlings, moisturizing and pollution prevention in the study. Firstly, put the tissue culture P.davidiana×P.bolleana seedlings in large test tubes with liquid medium for rooting for 12-15 d. Secondly, refine hydroponic seedlings in the aseptic water for 2 d. Then, transplant the seedlings into soil and cover with plastic cups to moisturizing, which will be removed after 15 d. At last, the 17 d old seedlings with soil were transplanted into the field. Using the above method, the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic, simple, small-scaled and high efficient but also suitable for many other woody plants, so it is easy to generalize.

Key words: Populus davidiana×, P.bolleana, tissue culture, rooting, transplanting, survival rate

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