短期盐胁迫下盐穗木的转录组分析

doi:10.7525/j.issn.1673-5102.2018.01.011

植物研究 ›› 2018, Vol. 38 ›› Issue (1): 91-99.doi: 10.7525/j.issn.1673-5102.2018.01.011

短期盐胁迫下盐穗木的转录组分析

张丽丽^1,2, 张富春^1,2

1. 新疆大学生命科学与技术学院, 乌鲁木齐 830046;
2. 新疆生物资源基因工程重点实验室, 乌鲁木齐 830046

收稿日期:2017-05-08 出版日期:2018-01-15 发布日期:2018-01-06
通讯作者: 张富春,E-mail:zfcxju@xju.edu.cn E-mail:zfcxju@xju.edu.cn
作者简介:张丽丽(1993—),女,硕士研究生,主要从事植物分子生物学研究.
基金资助:
新疆重点实验室专项资金资助项目（2014KL001）

Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress

ZHANG Li-Li^1,2, ZHANG Fu-Chun^1,2

1. College of Life Science and Technology, Xinjiang University, Urumqi 830046;
2. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046

Received:2017-05-08 Online:2018-01-15 Published:2018-01-06
Supported by:
Supported by Special Funds of Xinjiang Key Laboratory(2014KL001)

摘要/Abstract

摘要： 盐穗木（Halostachys caspica）是荒漠盐碱地广泛分布的盐生植物，具有极强的耐盐性。为揭示盐胁迫下盐穗木基因组层面的基因表达变化特性，通过对300和500mmol·L^-1 NaCl胁迫3h的盐穗木同化枝进行了转录组测序。有效序列组装共得到153298条平均长度为643bp的unigenes，进行GO和KEGG功能聚类，分别获得47个GO功能小类和118个KEGG通路。差异表达基因分析显示，短期低盐（300mmol·L^-1）响应基因有4432个，高盐（500mmol·L^-1）响应基因有2580个，两个胁迫的共差异基因有1245个，主要富集在细胞过程、代谢过程和响应刺激等类别中。从短期盐胁迫下盐穗木转录组筛选出渗透调节和活性氧清除的相关基因，大多为上调基因。说明盐穗木能够通过促进渗透调节和增强活性氧清除提高短期的盐胁迫适应能力。

关键词: 盐穗木, 盐胁迫, 转录组, 差异基因, 渗透调节, 活性氧

Abstract: As a halophyte with strong salt tolerance, Halostachys caspica widely distributes in desert saline-alkali land. In order to reveal the genomic changes of gene expression under salt stress, transcriptome sequencing of H.caspica assimilating branches with treatments of 300 and 500 mmol·L^-1 NaCl for 3 h was performed. A total of 153 298 unigenes with an average length of 643 bp were obtained with clean reads assembled. The 47 subclasses and 118 KEGG pathways were enriched in the GO terms and KEGG pathways, respectively. Differentially expressed genes analysis showed that there were 4 432 and 2 580 unigenes in response to low salt (300 mmol·L^-1) and high salt (500 mmol·L^-1) in short-term stress, respectively. The 1 245 unigenes were the common differentially expressed genes in the two salt stresses. They were mainly enriched in cellular process, metabolic process and response to stimulus. The osmotic regulation and reactive oxygen species (ROS) scavenging genes were screened out, and most of them were up-regulated. Therefore, H.caspica could improve short-term salt stress adaptation by enhancing the osmotic adjustment and ROS scavenging.

Key words: Halostachys caspica, salt stress, transcriptome, differentially expressed genes, osmotic adjustment, reactive oxygen species

中图分类号:

S687

张丽丽, 张富春. 短期盐胁迫下盐穗木的转录组分析[J]. 植物研究, 2018, 38(1): 91-99.

ZHANG Li-Li, ZHANG Fu-Chun. Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress[J]. Bulletin of Botanical Research, 2018, 38(1): 91-99.

参考文献

1. Zhu J K. Plant salt tolerance[J]. Trends in Plant Science,2001,6(2):66-71.
2. Hasegawa P M,Bressan R A,Zhu J K,et al. Plant cellular and molecular responses to high salinity[J]. Annual Review of Plant Physiology and Plant Molecular Biology,2000,51:463-499.
3. Sahu B B,Shaw B P. Isolation,identification and expression analysis of salt-induced genes in Suaeda maritima,a natural halophyte,using PCR-based suppression subtractive hybridization[J]. BMC Plant Biology,2009,9:69.
4. Ventura Y,Eshel A,Pasternak D,et al. The development of halophyte-based agriculture:past and present[J]. Annals of Botany,2014,115(3):529-540.
5. 郗金标,张福锁,毛达如,等. 新疆盐生植物群落物种多样性及其分布规律的初步研究[J]. 林业科学,2006,42(10):6-12.Xi J B,Zhang F S,Mao D R,et al. Species diversity and distribution of halophytic vegetation in Xinjiang[J]. Scientia Silvae Sinicae,2006,42(10):6-12.
6. Kumari S,Sabharwal V P N,Kushwaha H R,et al. Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L. [J]. Functional & Integrative Genomics,2009,9(1):109-123.
7. Xie Q,Niu J,Xu X L,et al. De novo assembly of the Japanese lawngrass(Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress[J]. Frontiers in Plant Science,2015,6:610.
8. Jin H X,Dong D K,Yang Q H,et al. Salt-responsive transcriptome profiling of Suaeda glauca via RNA sequencing[J]. PLoS One,2016,11(3):e0150504.
9. 杜驰,张冀,张富春. 盐胁迫诱导盐穗木Rev1、Rev3基因的表达分析[J]. 植物研究,2017,37(2):211-215,226.Du C,Zhang J,Zhang F C. Expression analysis of Rev1 and Rev3 of Halostachys caspica under salt stress[J]. Bulletin of Botanical Research,2017,37(2):211-215,226.
10. Türkan I,Demiral T. Recent developments in understanding salinity tolerance[J]. Environmental and Experimental Botany,2009,67(1):2-9.
11. Deinlein U,Stephan A B,Horie T,et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science,2014,19(6):371-379.
12. Benhassaine-kesri G,Aid F,Demandre C,et al. Drought stress affects chloroplast lipid metabolism in rape(Brassica napus) leaves[J]. Physiologia Plantarum,2002,115(2):221-227.
13. Konova I V,Sergeeva Y E,Galanina L A,et al. Lipid synthesis by Geomyces pannorum under the impact of stress factors[J]. Microbiology,2009,78(1):42-47.
14. Miled D D B,Zarrouk M,Chérif A. Sodium chloride effects on lipase activity in germinating rape seeds[J]. Biochemical Society Transactions,2000,28(6):899-902.
15. Stitt M,Sulpice R,Keurentjes J. Metabolic networks:how to identify key components in the regulation of metabolism and growth[J]. Plant Physiology,2010,152(2):428-444.
16. Ritala A,Dong L M,Imseng N,et al. Evaluation of tobacco(Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol,the first committed step in terpenoid indole alkaloid pathway[J]. Journal of Biotechnology,2014,176:20-28.
17. Li C Y,Leopold A L,Sander G W,et al. The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway[J]. BMC Plant Biology,2013,13(1):155.
18. 赵航,贾富强,张富春,等. 盐胁迫下盐穗木差异表达基因的转录组信息分析[J]. 生物信息学,2014,12(2):90-98.Zhao H,Jia F Q,Zhang F C,et al. The transcriptome information analysis of differentially expressed genes of Halostachys caspica under salt stress[J]. Chinese Journal of Bioinformatics,2014,12(2):90-98.
19. 黎裕. 植物的渗透调节与其它生理过程的关系及其在作物改良中的应用[J]. 植物生理学通讯,1994,30(5):377-383.Li Y. The relationship between osmotic adjustment and other physiological processes and its application in crop improvement[J]. Plant Physiology Communications,1994,30(5):377-383.
20. Hanson A D,Nelsen C E,Pedersen A R,et al. Capacity for proline accumulation during water stress in barley and its implications for breeding for drought resistance[J]. Crop Science,1979,19(4):489-493.
21. Patakas A,Nikolaou N,Zioziou E,et al. The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines[J]. Plant Science,2002,163(2):361-367.
22. 王娟,李德全. 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报,2001,18(4):459-465.Wang J,Li D Q. The accumulation of plant osmoticum and activated oxygen metabolism under stress[J]. Chinese Bulletin of Botany,2001,18(4):459-465.
23. Caprioli M,Katholm A K,Melone G,et al. Trehalose in desiccated rotifers:a comparison between a bdelloid and a monogonont species[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2004,139(4):527-532.
24. Zhang H M,Murzello C,Sun Y,et al. Choline and osmotic-stress tolerance induced in Arabidopsis by the soil microbe Bacillus subtilis(GB03)[J]. Molecular Plant-Microbe Interactions,2010,23(8):1097-1104.
25. Suzuki N,Koussevitzky S,Mittler R,et al. ROS and redox signalling in the response of plants to abiotic stress[J]. Plant,Cell & Environment,2012,35(2):259-270.
26. Gill S S,Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry,2010,48(12):909-930.
27. Jaleel C A,Riadh K,Gopi R,et al. Antioxidant defense responses:physiological plasticity in higher plants under abiotic constraints[J]. Acta Physiologiae Plantarum,2009,31(3):427-436.
28. Chen S,Polle A. Salinity tolerance of Populus[J]. Plant Biology,2010,12(2):317-333.
29. Vaidyanathan H,Sivakumar P,Chakrabarty R,et al. Scavenging of reactive oxygen species in NaCl-stressed rice(Oryza sativa L.)-differential response in salt-tolerant and sensitive varieties[J]. Plant Science,2003,165(6):1411-1418.

[1]	隋德宗, 王保松. 盐胁迫下乌桕无性系叶片的比较蛋白组学研究[J]. 植物研究, 2023, 43(5): 679-689.
[2]	倪馨宇, 贺俊英, 燕孟娇, 杜超. RNA-Seq技术在珍稀濒危植物研究中的应用进展[J]. 植物研究, 2023, 43(4): 481-492.
[3]	徐磊, 胥晓, 刘沁松. 外源水杨酸对盐胁迫下珙桐幼苗抗氧化系统和基因表达的影响[J]. 植物研究, 2023, 43(4): 572-581.
[4]	郑占敏, 商玉冰, 周广波, 肖迪, 刘轶, 由香玲. PsnHB13与PsnHB15在小黑杨中的遗传转化与功能分析[J]. 植物研究, 2023, 43(3): 340-350.
[5]	廖诗贤, 王宇婷, 董立本, 顾咏梅, 贾丰璘, 姜廷波, 周博如. 小黑杨转录因子PsnbZIP1应答盐胁迫功能分析[J]. 植物研究, 2023, 43(2): 288-299.
[6]	刘森尧, 贾丰璘, 国庆, 樊高锋, 周博如, 姜廷波. 小黑杨转录因子PsnbHLH162基因在盐和低温胁迫下应答分析[J]. 植物研究, 2023, 43(2): 300-310.
[7]	杨洪, 王立丰, 代龙军, 郭冰冰. 死皮对橡胶树树皮线粒体超微结构及活性氧代谢的影响[J]. 植物研究, 2023, 43(1): 69-75.
[8]	岳莉然, 刘颖婕, 刘晨旭, 周蕴薇. 响应盐胁迫调控的露地菊miR398a的克隆及功能研究[J]. 植物研究, 2022, 42(6): 986-996.
[9]	李登高, 林睿, 穆青慧, 周娜, 张焱如, 白薇. 马铃薯StNPR4基因的克隆与功能分析[J]. 植物研究, 2022, 42(5): 821-829.
[10]	陈娇娆, 续旭, 胡章立, 杨爽. 植物感受盐胁迫及相关钙信号的研究进展[J]. 植物研究, 2022, 42(4): 713-720.
[11]	程赫, 田双慧, 张洋, 刘聪, 夏德安, 魏志刚. 毛果杨nsLTP基因家族全基因组水平鉴定及其表达特性分析[J]. 植物研究, 2022, 42(3): 412-423.
[12]	黄东梅, 陈颖, 白露, 倪迪安, 徐奕扬, 张志国, 秦巧平. 萱草叶片响应低温胁迫的转录组分析[J]. 植物研究, 2022, 42(3): 424-436.
[13]	刘建新, 刘瑞瑞, 刘秀丽, 贾海燕, 卜婷, 李娜. 不同时期喷施NaHS对盐碱胁迫下裸燕麦H₂S产生和活性氧代谢的影响[J]. 植物研究, 2022, 42(3): 455-465.
[14]	余静雅, 夏铭泽, 徐浩, 张发起. 青藏高原地区3种蒿属植物转录组比较分析[J]. 植物研究, 2022, 42(2): 200-210.
[15]	代雅琦, 刘艳萍, 韩路, 王海珍. 地下水埋深对胡杨叶片光合作用及抗氧化物质积累的影响[J]. 植物研究, 2022, 42(2): 299-308.

短期盐胁迫下盐穗木的转录组分析

Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价