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植物研究 ›› 2011, Vol. 31 ›› Issue (3): 271-276.doi: 10.7525/j.issn.1673-5102.2011.03.004

• 论文 • 上一篇    下一篇

桔梗花粉萌发与花粉管生长研究

刘自刚1;呼天明2;杨亚丽1;佘小平3;汪玉琳1;薛粉丽1;刘凤霞1   

  1. 1.商洛学院生物医药工程系,商洛 726000;2.西北农林科技大学动物科学学院,杨凌 712100;3.陕西师范大学生命科学学院,西安 710062
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 基金资助:
     

Pollen Germination and Growth of Platycodon grandiflorum(Jacq.)A.DC

LIU Zi-Gang;HU Tian-Ming;YANG Ya-Li;SHE Xiao-Ping;WANG Yu-Lin;XUE Fen-Li;LIU Feng-Xia   

  1. 1.Biological Medicine Engineering Department of Shangluo University,Shangluo 726000;2.College of Animal Science and Technology,Northwest A&F university,Yangling 721000;3.College of Life Science,Shanxi Normal University,Xi’an 710062
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Supported by:
     

摘要: 以2年生桔梗植株为材料,采用液体培养法研究了培养基种类、PEG、蔗糖、pH以及培养温度、培养时间对桔梗花粉离体萌发生长的影响,结果表明:(1)浓度为100~150 g·L-1的PEG可显著促进桔梗花粉萌发和花粉管的生长;200~250 g·L-1 PEG显著促进花粉萌发,但对花粉管生长的作用不显著。(2)100 g·L-1的蔗糖有利于花粉萌发和花粉管生长,高浓度蔗糖(200 g·L-1)有明显抑制作用;(3)桔梗花粉离体萌发和花粉管生长的适宜培养基为ME3+BK+10%蔗糖+150 g·L-1 PEG(pH5.8);(4)25~40℃条件下桔梗花粉均可较好萌发,以30℃培养1.5 h为最佳培养条件。

关键词: 桔梗, 花粉, 离体萌发, 花粉管生长

Abstract: Two-year-old Platycodon grandiflorum(Jacq.)A.DC trees grown in the experiment field of Xiangju Pharmacy Limited Company were used as the materials. The effects of culture medium, sucrose, PEG, pH value, culture time and temperature on the pollen germination and tube growth of P.grandiflorum were investigated using the method of liquid culture. The results showed that: (1)the concentration of 100-150 g·L-1 PEG could accelerate pollen germination and the growth of pollen tube, the range of 200-250 g·L-1 PEG could remarkably accelerate pollen germination, however, its function on the growth of pollen tube was not distinct. (2)100 g·L-1 sucrose was helpful for the pollens to germinate and grow, however, high concentration of sucrose can would strongly inhibit pollen germination and the growth of pollen tubes. (3)the optimum culture medium for pollen germination and growth of Platycodon were ME3+BK+10% sucrose+150 g·L-1 PEG (pH 5.8). (4)pollen germination was better at 25-40℃, and it was best at 30℃ for 1.5 h.

Key words: Platycodon grandiflorum, pollen, germination In vitro, growth of pollen tubes

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