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植物研究 ›› 2015, Vol. 35 ›› Issue (5): 710-715.doi: 10.7525/j.issn.1673-5102.2015.05.012

• 论文 • 上一篇    下一篇

胞外H2O2及NADPH氧化酶参与了铜胁迫对植物细胞死亡的诱导

冯汉青;白晶月;管冬冬;贾凌云;孙坤   

  1. 西北师范大学生命科学学院,兰州 730070
  • 出版日期:2015-09-15 发布日期:2015-11-20
  • 基金资助:
     

Extracellular H2O2 and NADPH Oxidase are Involved in the Copper-Induced Cell Death

FENG Han-Qing;BAI Jing-Yue;GUAN Dong-Dong;JIA Ling-Yun;SUN Kun   

  1. College of Life Sciences,Northwest Normal University,Lanzhou 730070
  • Online:2015-09-15 Published:2015-11-20
  • Supported by:
     

摘要: 以烟草悬浮细胞BY-2(Nicotiana tabacum L.cv.Bright Yellow-2)为材料,探讨了在铜离子胁迫下植物细胞死亡发生过程中胞外H2O2及NADPH氧化酶所扮演的角色。实验结果表明,随着外源CuCl2浓度的上升(从0~700 μmol·L-1),细胞死亡水平不断上升,且胞外H2O2的水平也不断增加。在300 μmol·L-1的CuCl2诱导细胞死亡的过程中,加入H2O2清除剂N-N-二甲基硫脲(DMTU)降低了胞外CuCl2胁迫下H2O2含量增加的同时也降低了细胞死亡水平的上升,这一观察表明了铜离子胁迫所导致的细胞死亡的发生和胞外H2O2的增加有关。进一步的研究表明,300 μmol·L-1 CuCl2的胁迫导致了NADPH氧化酶活性的显著性上升,而加入NADPH氧化酶的抑制剂(二亚苯基碘,DPI,)则降低了CuCl2胁迫所导致的细胞死亡和胞外H2O2含量的上升。上述结果表明,胞外H2O2和NADPH氧化酶参与了CuCl2对植物细胞死亡的诱导作用。

关键词: 细胞死亡, 铜离子, 胞外H2O2, NADPH氧化酶

Abstract: By using the tobacco (Nicotiana tabacum cv. Bright Yellow-2) cell suspension culture, we studied the roles of extracellular H2O2 and NADPH oxidase in the copper-induced cell death. With the increase of exogenous CuCl2 from 0 to 700 μmol·L-1, the level of cell death were increased followed by the increase of extracellular H2O2. In the cell subjected to 300 μmol·L-1 CuCl2, the addition of exogenous dimethyl thiourea (DMTU, a scavenger of H2O2) alleviated the increases of both the cell death level and the production of extracellular H2O2, which were induced by CuCl2(300 μmol·L-1). The copperinduced increase in the cell death was associated with the increase of extracellular H2O2. The CuCl2(300 μmol·L-1) stress induced an increase of the activity of NADPH oxidase. The addition of diphenylene iodonium (DPI, an inhibitor of NADPH oxidase) alleviated the increases of both the cell death level and the production of extracellular H2O2, which were induced by CuCl2 (300 μmol·L-1). Our results indicate that extracellular H2O2 and NADPH oxidase are involved in the copper-induced cell death.

Key words: cell death, copper, extracellular H2O2, NADPH oxidase

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