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植物研究 ›› 2015, Vol. 35 ›› Issue (4): 623-627.doi: 10.7525/j.issn.1673-5102.2015.04.023

• 论文 • 上一篇    下一篇

碳源对迷迭香悬浮培养细胞的生长、迷迭香酸积累及抗氧化酶活性的影响

曲丹;王慧梅*;任洁   

  1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040
  • 出版日期:2015-07-20 发布日期:2016-03-09
  • 基金资助:
     

Effects of Sugar content on Cell Growth,Accumulation of Rosemary Acid, and Antioxidative Enzyme Activity in Suspension Cells of Rosemary

QU Dan;WANG Hui-Mei*;REN Jie   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Online:2015-07-20 Published:2016-03-09
  • Supported by:
     

摘要: 以迷迭香悬浮培养细胞为材料,详细研究了基本培养基中添加蔗糖、麦芽糖和葡萄糖对细胞生长及次生代谢产物积累的影响,同时对不同蔗糖浓度处理的悬浮培养细胞抗氧化酶活性进行了研究。研究结果表明:在不同的糖处理中,30 g·L-1的蔗糖、70 g·L-1的麦芽糖及40 g·L-1的葡萄糖最有利于迷迭香悬浮培养细胞生长。30 g·L-1蔗糖和70 g·L-1麦芽糖处理中悬浮培养细胞的生长率分别为74.08%和72.33%,高出40 g·L-1葡萄糖处理接近3倍之多。30 g·L-1蔗糖处理的悬浮培养细胞迷迭香酸含量高出70 g·L-1麦芽糖处理228倍,略低于40 g·L-1葡萄糖处理。在不同蔗糖的处理中,随着蔗糖浓度的增加,迷迭香酸含量均呈现增加趋势,表明高浓度的蔗糖有利于悬浮培养细胞迷迭香酸的积累。在高浓度的蔗糖处理中,悬浮培养细胞H2O2和MDA含量明显增加,同时抗氧化酶SOD、POD及CAT的活性也明显增强,表明高浓度的蔗糖产生了渗透胁迫,这种渗透胁迫虽不利于迷迭香悬浮培养细胞的生长,但有利于次生代谢产物的积累。综合迷迭香悬浮细胞的生长率和迷迭香酸的含量,我们最终得出30 g·L-1的蔗糖最有利于迷迭香悬浮细胞的培养。

关键词: 糖, 悬浮培养细胞, 生长, 次生代谢产物, 抗氧化酶活性

Abstract: We investigated the effects of different concentrations of sucrose, maltose and glucose on cell growth, rosemary acid accumulation in rosemary suspension cells. And we also studied the different concentration of sucrose on antioxidant enzyme activity in rosemary suspension cells. The optimum sugar treatment for cell biomass accumulation was 30 g·L-1 sucrose, 70 g·L-1 maltose and 40 g·L-1 glucose in rosemary suspension cells. Moreover, the growth rates of suspension culture cells were 74.08% and 72.33% under processing of 30 g·L-1 sucrose and 70 g·L-1 maltose, respectively,but was nearly three times more than that of 40 g·L-1 glucose. The content of rosemary acid in 30 g·L-1 sucrose suspension culture cells was 228 times more than that in 70 g·L-1 maltose, but slightly lower than that in 40 g·L-1 glucose. Under different sucrose treatment, rosemary acid content showed an increase trend with the increase of sucrose content, which meant that a high concentration of sucrose was conducive to rosemary acid accumulation. H2O2 and MDA content obviously increased under the treatment of higher sucrose concentration, while antioxidant enzymes such as catalase(CAT), guaiacol peroxidase(G-POD) and SOD also increased. The osmotic stress under high concentration of sucrose was bad for the growth of rosemary suspension culture cells, but was conducive to the accumulation of secondary metabolites. Considering the growth rate and rosemary acid content, the medium supplemented with 30 g·L-1 sucrose was most suitable for rosemary suspension cultured cells.

Key words: sucrose, suspension cells, growth, secondary metabolite, antioxidant enzyme

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