• 研究报告 •

### 基于ISSR分子标记的孑遗濒危植物四合木遗传结构分析

1. 榆林学院，陕西省陕北生态修复重点实验室，榆林 719000
• 收稿日期:2019-12-19 出版日期:2021-05-20 发布日期:2021-03-24
• 作者简介:段义忠（1981—），男，副教授，博士，主要从事植物谱系地理研究。
• 基金资助:
国家自然科学基金项目(41601059)

### Genetic Diversity of Plant Tetraenamongolica by ISSR Markers

Yi-Zhong DUAN, Hai-Tao WANG, Ge-Ge ZHANG, Wei-Na YAN

1. Shaanxi Key Laboratory of Ecological Restoration in Northern Shaanxi Mining Area，Yulin University，Yulin 719000
• Received:2019-12-19 Online:2021-05-20 Published:2021-03-24
• About author:DUAN Yi-Zhong(1981—),male,associate professor,research mainly focuses on plant phylogeography．
• Supported by:
National Natural Science Foundation of China(41601059)

Abstract:

Tetraena mongolica is an endangered plant belonging to a single genus， known as the ‘plant giant panda’. It has been included in the first batch of national rare and endangered plant red papers. It is a special shrub endemic to the west of Ordos Plateau and its distributed area is small. Six different geographical populations of T.mongolica were used as test materials. Six of the 30 UBC primers were screened for the most suitable primers， and the ISSR amplification reaction system and amplification procedure were reasonably optimized. The 370 bands were amplified from the primers， and 71% of T.mongolica was polymorphic bands. The results of the analysis can be shown as follows： ①The Nei’s gene diversity index was 0.316 8 and Shannon’s polymorphism information index was 0.458 6. The genetic diversity levels of T.mongolica different provenances were little different； ②The percentage of groups polymorphism allele in the population ranged from 70.00%-83.33%， the Nei’s genetic diversity index was 0.286 5-0.350 8， and the Shannon’s polymorphism information index was 0.423 6-0.504 9. The Gst of genetic differentiation coefficient between the six populations was 0.125 3， the results explain that there is a certain degree of genetic differentiation among populations； ③The gene flow of the six populations was 3.491 5>1， indicating that there was a certain gene flow among the six populations， which could prevent the genetic differentiation caused by genetic drift； ④Cluster analysis was used to classify the tetrahedrons of six different geographic populations into three groups. The populations of Qianligou， Yikebulage， and Balagong clustered into the first category， and then they were clustered with the Dengkou-taositu rabbits into the second category. There are two major categories， and the population of Natural Reserve of T.mongolica and the population Gandle Mountain constituted the third largest category. This shows that distance factors are the main factors that affect genetic differentiation among T.mongolica populations， but different habits conditions also have an impact on the genetic differentiation of T.mongolica populations.