四川省不同天然种群珙桐叶片解剖结构与其耐热性的关系

doi:10.7525/j.issn.1673-5102.2019.02.006

摘要/Abstract

摘要： 以四川省15个珙桐种群为试材，采用石蜡切片法、NaClO法观察叶片16项解剖结构，应用变异系数、聚类分析、相关性分析对叶片解剖结构进行筛选，利用隶属函数法对不同种群的耐热性进行综合评价，以期了解珙桐应对热胁迫的生理变化机制，为野生珙桐资源的保护及园林应用提供参考。结果表明：珙桐叶片为异面叶，气孔主要分布于下表皮。16项解剖结构指标在不同种群间达到极显著差异水平；栅海比、栅栏组织厚度、气孔密度、下表皮角质厚度、主脉凸起度、上表皮角质层厚度、下表皮细胞厚度是影响珙桐耐热性的主要解剖结构指标，叶厚度、海绵组织厚度、主脉厚度、上表皮细胞厚度、主脉维管束厚度、气孔长度、气孔宽度、叶片结构紧密度、叶片结构疏松度与耐热性关系不大；15个珙桐种群耐热性存在一定差异，其强弱顺序为：汉源 > 筠连 > 雷波 > 沐川 > 屏山 > 荥经 > 天全 > 美姑 > 平武 > 碧峰峡 > 北川 > 洪雅 > 宝兴 > 大邑 > 邛崃；15个种群耐热性大致分为4类：汉源种群为耐热型，筠连、雷波、沐川等3个种群为中度耐热型，屏山、荥经、天全、美姑、平武、碧峰峡、北川为低耐热型，洪雅、宝兴、雷波、邛崃等4个种群为不耐热型。

关键词: 珙桐, 天然种群, 耐热性, 解剖结构

Abstract: In order to understand the heat resistance mechanism and the level of heat resistance of Dove tree(Davidia involucrata) populations in Sichuan Province, 16 anatomical structures of 15 representative natural populations in Sichuan Province were investigated by paraffin section and NaClO. Moreover, coefficient of variation, correlation analysis, and cluster analysis were used to screen representative structures. The 16 anatomical structure indexes all reached a significant level difference among 15 natural populations(P<0.01), thickness ratio of palisade and spongy tissue, thickness of palisade tissue, stoma density, cuticle thickness of lower epidermis, protuberant degree of main vein, cuticle thickness of upper epidermis, cuticle thickness of upper epidermis and thickness of lower epidermis were the main factors related to the heat resistance, while other indexes, such as leaf thickness, sponge tissue thickness, thickness of main vein, thickness of epidemic cell, thickness of vascular bundle in main vein stoma length, stoma width, tightness of leaf structure and looseness of leaf structures did not show much effect on heat resistance. There were some differences among 15 populations in heat resistance, and the descending order was Hanyuan, Junlian, Leibo, Muchuan, Pingshan, Yingjing, Tianquan, Meigu, Pingwu, Bifengxia, Beichuan, Hongya, Baoxing, Dayi, Qionglai. According to the heat hardiness, these populations could be divided into four groups, Hanyuan; Jun Lian, Leibo and Muchuan; Pingshan, Yingjing, Tianquan, Meigu, Pingwu, Bifengxia, Beichuan LR; and Hongya, Baoxing, Dayi, Qionglai.

Key words: Davidia involucrata, natural populations, heat resistance, anatomic structures

中图分类号:

S719

张腾驹, 陈小红, 刘静, 康喜坤. 四川省不同天然种群珙桐叶片解剖结构与其耐热性的关系[J]. 植物研究, 2019, 39(2): 208-221.

ZHANG Teng-Ju, CHEN Xiao-Hong, LIU Jing, KANG Xi-Kun. Relationship between Anatomical Structures and Heat Resistance of Davidia involucrata Natural Populations in Sichuan Province[J]. Bulletin of Botanical Research, 2019, 39(2): 208-221.

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