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植物研究 ›› 2010, Vol. 30 ›› Issue (3): 283-288.doi: 10.7525/j.issn.1673-5102.2010.03.006

• 论文 • 上一篇    下一篇

水稻57H突变体系列的胚乳储藏蛋白质表型多样性及其分类

田怀东1;张变香2   

  1. 1.山西大学生命科学学院,太原 030006;2.山西大学化学化工学院,太原 030006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:
     

Diversity of Phenotypes of Endosperm Storage Proteins of Rice 57H Mutants and Their Classification

TIAN Huai-Dong;ZHANG Bian-Xiang   

  1. 1.College of Life Science,Shanxi University,Taiyuan 030006;2.College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Supported by:
     

摘要: 种子储藏蛋白质主要由谷蛋白、醇溶谷蛋白和球蛋白组成。这些蛋白质在种子发育期被合成后,经过区室化过程,被蓄积在胚乳中。本研究系统分析了水稻57H突变体的表型多样性,旨在为胚乳储藏蛋白质的遗传调节机制的全面解明展示一个新的前景。胚乳蛋白质的SDS-PAGE分析和免疫印迹分析显示了高量含有57 kD谷蛋白前体的水稻57H突变体系列具有多样的储藏蛋白质表型。基于其表型的多样性,57H突变体系列被分成了三种类型。与野生型水稻品种相比,所有的类型Ⅰ突变体(glup4,glup6,Glup5,esp2)不仅高量蓄积谷蛋白前体、少量蓄积成熟型谷蛋白的40 kD酸性亚基和20 kD碱性亚基,而且显著减少了13 kD醇溶谷蛋白b组分和26 kDa球蛋白的蓄积;类型Ⅱ突变体(Glup1,glup2)不仅高量蓄积谷蛋白前体,还减少了26 kDa球蛋白的蓄积;类型Ⅲ突变体(glup3)除了高量蓄积谷蛋白前体、少量蓄积成熟型谷蛋白亚基之外,并没有减少其它储藏蛋白质的蓄积。结果指出了57H变异系列对储藏蛋白的蓄积具有多样的影响,实质上是关于储藏蛋白质区室化的遗传体系。并就该遗传体系对储藏蛋白质的翻译后区室化及其营养性状的可能的影响进行了讨论。

关键词: 水稻, 57H突变体, 储藏蛋白质, 多样性

Abstract: Seed storage proteins are mainly composed of glutelins, prolamins and globulins. The proteins are synthesized and compartmented during seed development. Compartmentation of the proteins results in their accumulation. This study was performed to present a novel perspective for overall understanding of genetic mechanism regulating the protein compartmentation in rice endosperm. SDS-PAGE and immunoblot analyses of the storage proteins showed that rice 57H mutants containing the high amount of 57 kD glutelin precursor had the various phenotypes of endosperm storage proteins, in comparison with the wild type of cultivar. The mutants were classified into 3 types on the basis of the diversity of their phenotypes. The typeⅠ of glup4, glup6, Glup5 and esp2 mutants all showed not only the deposition of the glutelin precursor and the inhibited deposition of the mature types of 40 kD and 20 kD glutelin subunits, but also the decreased depositions of 26 kD globulin and b species of 13 kD prolamins. The type Ⅱ of Glup1 and glup2 mutants showed the decreased depositions of 26 kD globulin in addition to the glutelin precursor deposition. The type Ⅲ of glup3 mutant only showed the glutelin precursor deposition following the inhibited deposition of the glutelin subunits. These results indicate that 57H mutations have various effects on the accumulation of the storage proteins, and thus are the essentially genetic systems concerning the protein compartmentation. The possible effects of the genetic systems on the post-translational compartmentation and nutritional properties of rice storage proteins are discussed in this paper.

Key words: Oryza Sativa L., 57H Mutants, Storage Proteins, Diversity

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