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植物研究 ›› 2020, Vol. 40 ›› Issue (5): 775-781.doi: 10.7525/j.issn.1673-5102.2020.05.017

• 研究简报 • 上一篇    

响应面优化超声辅助法提取拐枣种子中二氢杨梅素的工艺

张晓南1,2, 朱鸿维2, 赵善舶2, 白晨2, 石雅楠2, 徐浩朋2, 尹恋1, 胡淑敏1, 尹花1   

  1. 1. 青岛啤酒股份有限公司, 啤酒生物发酵工程国家重点实验室, 青岛 266071;
    2. 东北农业大学食品学院, 哈尔滨 150036
  • 收稿日期:2019-09-26 发布日期:2020-07-10
  • 通讯作者: 尹花,E-mail:xiaonanzhang@neau.edu.cn E-mail:xiaonanzhang@neau.edu.cn
  • 作者简介:张晓南(1982-),男,副教授,主要从事方向为食品化学方面的研究。
  • 基金资助:
    黑龙江省自然基金面上项目(C2018025)

Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb. Using Response Surface Methodology

ZHANG Xiao-Nan1,2, ZHU Hong-Wei2, ZHAO Shan-Bo2, BAI Chen2, SHI Ya-Nan2, XU Hao-Peng2, YIN Lian1, HU Shu-Min1, YIN Hua1   

  1. 1. State key laboratory of beer biological fermentation engineering, Qingdao beer co., LTD., Qingdao 266071;
    2. Food Science College, Northeast Agricultural University, Harbin 150036
  • Received:2019-09-26 Published:2020-07-10
  • Supported by:
    Heilongjiang Natural Fund General Project(C2018025)

摘要: 二氢杨梅素和杨梅素是拐枣种子中的重要成分。通过超声辅助法从拐枣种子中提取二氢杨梅素,采用单因素法考察乙醇体积分数、超声辐照功率、提取温度、液料比和超声辐照时间影响参数的基础上,并选用Box-Behnken响应面设计法分析建立了超声辐照功率、超声辐照时间和液料比的二次多项式模型,优化提取工艺。结果得到超声辅助法提取拐枣种子中二氢杨梅素的最佳工艺参数为:乙醇体积分数为60%、超声辐照功率140 W、超声辐照时间30 min、液料比20.5 mL·g-1,提取温度40℃。在此最佳条件下,二氢杨梅素得率为2.14±0.09 mg·g-1。本提取方法简单快速,效率高,有利于拐枣资源的综合加工利用。

关键词: 拐枣, 种子, 超声辅助提取, 二氢杨梅素, 响应面

Abstract: Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb. The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction, ultrasound irradiation power, extraction temperature, liquid-solid ratio and ultrasound irradiation time. The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power, ultrasound irradiation time and liquid-solid ratio, and optimized extraction process. The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%, ultrasonic irradiation power of 140 W, ultrasonic irradiation time of 30 min, liquid-solid ratio of 20.5 mL·g-1, and extraction temperature of 40℃. Under these optimal conditions, the yield of dihydromyricetin was 2.14±0.09 mg·g-1. The extraction method is simple, rapid, and high in efficiency, which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.

Key words: Hovenia dulcis Thunb., seed, ultrasound-assisted extraction, dihydromyricetin, response surface methodology

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