全基因组分析杨树WOX基因家族在茎部发育中的作用

doi:10.7525/j.issn.1673-5102.2019.04.011

植物研究 ›› 2019, Vol. 39 ›› Issue (4): 568-577.doi: 10.7525/j.issn.1673-5102.2019.04.011

全基因组分析杨树WOX基因家族在茎部发育中的作用

王爽, 张洋, 任梦轩, 刘莹莹, 魏志刚

林木遗传育种国家重点实验室, 东北林业大学, 哈尔滨 150040

收稿日期:2019-03-05 出版日期:2019-07-05 发布日期:2019-07-03
通讯作者: 魏志刚 E-mail:zhigangwei@nefu.edu
作者简介:王爽(1993-),女,硕士研究生,主要从事植物次生细胞壁研究。
基金资助:
国家自然科学基金（31770640）资助

Genome-Wide Analysis of the WOX Family Reveals Their Involvement in Stem Growth of Populus trichocarpa

WANG Shuang, ZHANG Yang, REN Meng-Xuan, LIU Ying-Ying, WEI Zhi-Gang

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2019-03-05 Online:2019-07-05 Published:2019-07-03
Supported by:
National Natural Science Foundation of China(31770640)

摘要/Abstract

摘要： WUSCHEL-related homeobox （WOX）家族是植物特有的转录因子家族，参与分生组织细胞分裂分化、初生和次生物质代谢及植物激素信号转导等多个发育过程，目前尚未有从全基因组分析该基因家族参与杨树茎部发育的相关研究。本项研究旨在对杨树WOX基因家族进行鉴定，在杨树基因中发现18个WOX候选基因，将这些候选基因分为三组，同一分组的大多数WOX家族成员具有相似的基因结构和保守的基序。根据不同发育阶段茎部转录组数据，系统分析了WOX家族成员在茎部不同发育阶段的特异表达情况，并采用qRT-PCR对上述结果进行了验证。结果表明，杨树WOX基因家族在茎部不同发育阶段表现出不同的表达模式，为毛果杨WOX家族的功能研究与利用奠定基础。

关键词: 毛果杨, WOX基因, 转录因子, 表达模式

Abstract: The WUSCHEL-related homeobox(WOX) family is a plant-specific transcription factor family involved in multiple developmental processes including meristematic cell division and differentiation, primary and secondary biomass metabolism, and plant hormone signaling. At present, there is no relevant research on genome-wide analysis of the gene family involved in stem development of poplar. We identified the poplar WOX gene family, and found 18 WOX candidate genes in the poplar gene. These candidate genes were divided into three groups. Most of the WOX family members in the same group had similar gene structure and conservation motif. According to the transcriptome data of stems at different developmental stages, the specific expression of WOX family members in different developmental stages of stems was systematically analyzed, and the above results were verified by qRT-PCR. The poplar WOX gene family showed different expression patterns at different developmental stages of the stem, which laid a foundation for the functional research and utilization of the WOX family.

Key words: Populus trichocarpa, WOX gene, transcription factors, expression patterns

中图分类号:

S792.11

王爽, 张洋, 任梦轩, 刘莹莹, 魏志刚. 全基因组分析杨树WOX基因家族在茎部发育中的作用[J]. 植物研究, 2019, 39(4): 568-577.

WANG Shuang, ZHANG Yang, REN Meng-Xuan, LIU Ying-Ying, WEI Zhi-Gang. Genome-Wide Analysis of the WOX Family Reveals Their Involvement in Stem Growth of Populus trichocarpa[J]. Bulletin of Botanical Research, 2019, 39(4): 568-577.

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全基因组分析杨树WOX基因家族在茎部发育中的作用

Genome-Wide Analysis of the WOX Family Reveals Their Involvement in Stem Growth of Populus trichocarpa

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