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植物研究 ›› 2018, Vol. 38 ›› Issue (5): 669-674.doi: 10.7525/j.issn.1673-5102.2018.05.005

• 研究报告 • 上一篇    下一篇

NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响

赵晓菊1,2, 张丽霞1, 满秀玲2   

  1. 1. 大庆师范学院生物工程学院, 大庆 163712;
    2. 东北林业大学林学院, 哈尔滨 150040
  • 收稿日期:2018-04-08 出版日期:2018-09-15 发布日期:2018-08-31
  • 通讯作者: 满秀玲 E-mail:mannefu@163.com
  • 作者简介:赵晓菊(1978-),女,副教授,主要从事植物生理生态研究。
  • 基金资助:
    黑龙江省青年科学基金(QC2015042)

Effects of Exogenous NO on Seed Germination and Physiological Metabolism in Catharanthus roseus Seedling under NaCl Stress

ZHAO Xiao-Ju1,2, ZHANG Li-Xia1, MAN Xiu-Ling2   

  1. 1. Bioengineering Institute, Daqing Normal University, Daqing 163712;
    2. College of Forestry, Northeast Forestry University, Harbin 150040
  • Received:2018-04-08 Online:2018-09-15 Published:2018-08-31
  • Supported by:
    This study was financially supported by Natural Science Foundation of Heilongjiang province(QC2015042)

摘要: 为探讨NO对盐胁迫下长春花(Catharanthus roseus)种子萌发及幼苗生长的缓解作用,在50 mmol·L-1 NaCl胁迫下,研究了不同浓度外源NO供体硝普钠(SNP)处理对长春花种子萌发及幼苗生理代谢的影响。研究表明:盐胁迫抑制长春花种子萌发,0.1 mmol·L-1 SNP缓解盐胁迫对长春花种子萌发和幼苗生长效果最好,显著提高种子发芽率、发芽指数、活力指数和叶片中脯氨酸(Pro)含量,降低了丙二醛(MDA)含量,0.1~0.5 mmol·L-1 SNP下硝酸还原酶(NR)活性增强,大于0.5 mmol·L-1 SNP加重盐胁迫伤害。适当浓度的NO可有效缓解盐胁迫对长春花种子和幼苗的伤害。

关键词: 一氧化氮, 盐胁迫, 长春花, 种子萌发, 硝酸还原酶

Abstract: Under 50 mmol·L-1 NaCl stress condition, we studied the relaxation effects of sodium nitroprusside(SNP, an exogenous nitric oxide donor) at the contents of 0.05, 0.1, 0.5, 1.0 and 2.0 mmol·L-1 on germination and physiological metabolism in Catharanthus roseus seedlings. The results showed that NaCl stress inhibited the germination of C.roseus and SNP could alleviate its stress effect. At the 0.1 mmol·L-1 of SNP the seed germination, germination and vitality index, and contents of proline in leaves were increased significantly, while malondialdehyde(MDA) content in leaves were decreased dramatically. Nitrate reductase(NR) activities in 0.1-0.5 mmol·L-1 SNP groups reached to higher levels compared to that of control group. However, more than 0.5 mmol·L-1 SNP enhanced the stress effect of NaCl. Therefore, the 0.1 mmol·L-1 was the most appropriate content for SNP to alleviate NaCl stress. In conclusion, appropriate contents of exogenous NO could alleviate NaCl stress on C.roseus seeds and seedlings effectively.

Key words: Nitric oxide, salt stress, Catharanthus roseus, seed germination, nitrate reductase

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