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植物研究 ›› 2018, Vol. 38 ›› Issue (2): 298-305.doi: 10.7525/j.issn.1673-5102.2018.02.019

• 研究报告 • 上一篇    下一篇

黄芪种子萌发及萌发后生长过程中黄酮类化合物合成的动态变化

杨楠, 王曦, 郭晓瑞, 刘洋, 唐中华, 王洪政   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2017-10-15 出版日期:2018-03-15 发布日期:2018-03-23
  • 通讯作者: 王洪政 E-mail:hzwang@nefu.edu.cn
  • 作者简介:杨楠(1993-),女,硕士研究生,主要从事药用植物种子萌发及次生代谢研究。
  • 基金资助:
    中央高校基本科研业务费专项资金项目资助(2572017DA05);国家自然科学基金资助(31400337)

Variation in Flavonoids Biosynthesis during Seed Germination and Post-germination Growth in Astragalus membranaceus

YANG Nan, WANG Xi, GUO Xiao-Rui, LIU Yang, TANG Zhong-Hua, WANG Hong-Zheng   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2017-10-15 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572017DA05);National Natural Science Foundation of China(31400337)

摘要: 在对膜荚黄芪和蒙古黄芪种子萌发和萌发后生长时期进行划分的基础上,研究了黄芪中3种主要黄酮类化合物芒柄花素、毛蕊异黄酮和毛蕊异黄酮苷在萌发和萌发后生长过程中含量的变化。为了弄清黄酮含量变化的机制,我们还对黄芪中黄酮生物合成途径酶的转录水平做了研究。结果表明,在种子萌发和萌发后生长阶段,两种黄芪中均出现了明显的黄酮类化合物累积增加的情况。通过检测黄酮类物质生物合成途径酶基因的转录表达水平,我们发现萌发阶段蒙古黄芪中黄酮类物质积累增加主要由上游途径酶基因的表达水平升高引起,而膜荚黄芪中则几乎涉及到所有途径酶。此外,我们还发现萌发后生长阶段中,两种黄芪中合成途径酶基因转录对黄酮类物质含量的调控则选择性地存在时间延迟效应。

关键词: 黄芪, 萌发, 萌发后生长, 黄酮, 途径酶

Abstract: Based on the stage definitions of seed germination and post-germination growth, we investigated the variations of contents of three major flavonoids during the two stages in Astragalus membranaceus and its variety of mongholicus. Besides, in order to understand how the variations were induced, the transcriptions of pathway enzyme involving in flavonoid biosynthesis were profiled. The contents of flavonoids increased during the two stages in both A.membranaceus and the variety. With the detection of gene transcriptions of the pathway enzymes, only the enzymes in upstream of the pathway contributed to the increase of flavonoids in the variety of mongholicus during the germination, whereas it was that almost all the pathway enzymes were involved in A.membranaceus. The transcriptions of pathway enzymes regulated the accumulations of flavonoids during post-germination growth, selectively with a pattern of time delay.

Key words: Astragalus membranaceus, germination, post-germination growth, flavonoid, pathway enzymes

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