青藏高原东南部山丹nrDNA ITS和cpDNA petB/petD 序列的分子进化特点

doi:10.7525/j.issn.1673-5102.2018.01.014

植物研究 ›› 2018, Vol. 38 ›› Issue (1): 117-124.doi: 10.7525/j.issn.1673-5102.2018.01.014

青藏高原东南部山丹nrDNA ITS和cpDNA petB/petD 序列的分子进化特点

蒋福娟^1,3, 唐楠^1,2, 唐道城¹, 巨秀婷¹, 孙静清⁴

1. 青海大学高原花卉研究中心/青海省园林植物与观赏园艺重点实验室, 西宁 810016;
2. 青海省高原作物种质资源创新与利用国家重点实验室培育基地, 西宁 810016;
3. 大通县林业局, 大通 810100;
4. 西宁市人民公园, 西宁 810008

收稿日期:2017-05-24 出版日期:2018-01-15 发布日期:2018-01-06
通讯作者: 唐楠,E-mail:natasha_tn@hotmail.com E-mail:natasha_tn@hotmail.com
作者简介:蒋福娟(1986—),女,硕士研究生,助理工程师,主要从事园林植物遗传育种研究工作.
基金资助:
国家自然科学基金项目31660582（Genetic mapping of resistance gene for tulip breaking virus）；青海省“高端创新人才千人计划”；中国科学院“西部之光”人才培养计划

Characteristic of Molecular Evolution for Lilium pumilum Redouté on Southeast of the Qinghai-Tibetan Plateau using nrDNA ITS and cpDNA petB/petD Sequence Analysis

JIANG Fu-Juan^1,3, TANG Nan^1,2, TANG Dao-Cheng¹, JU Xiu-Ting¹, SUN Jing-Qing⁴

1. Plateau Flower Research Center of Qinghai University/The key laboratory of landscape plants of Qinghai Province, Xining 810016;
2. State Key Laboratory Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, Xining 810016;
3. Datong County Forestry Bureau, Datong 810100;
4. Xining People's Park, Xining 810008

Received:2017-05-24 Online:2018-01-15 Published:2018-01-06
Supported by:
Natural Science Foundation of China 31660582(Genetic mapping of resistance gene for tulip breaking virus);“Thousand Talents Program”of Qinghai Province;CAS“Light of West China”Program

摘要/Abstract

摘要： 采用改良CTAB法提取青藏高原东南部山丹25个居群所有个体的基因组DNA，选取核基因ITS和叶绿体petB/petD区域进行PCR扩增、纯化和测序。对所有序列对位排列，其中ITS序列总长696bp，变异位点有4处，共产生7种单倍型，变异位点百分率为0.72%，（G+C）含量60.4%；petB/petD序列总长616bp，仅1处变异位点和2种单倍型，变异位点百分率为0.16%，（G+C）含量34.6%。表明山丹中，petB/petD区域较ITS序列保守，变异速率较慢。对ITS序列单倍型进行失配分布和中性检验分析发现，山丹现有分布范围可能经历了近期居群小范围扩张，AMOVA分析发现山丹居群的遗传变异主要存在于居群内，N_ST >G_ST（P>0.01），表明山丹的遗传变异有着不显著的谱系地理结构。因此，山丹ITS序列适合该种的谱系地理学研究。

关键词: 青藏高原东南部, 山丹, ITS, petB/petD, 分子进化

Abstract: In this study, total DNA was extracted from Lilium pumilum on southeast of the Qinghai-Tibetan Plateau using modified CTAB method. Using genome DNA as template, nrDNA ITS and cpDNA petB/petD regions were amplified, purified and sequenced. By sequence alignment, it was known that length of nrDNA ITS sequence was 696 bp, of which 4 variable sites with a percentage of 0.72% were found., The G+C content of the ITS sequence was 60.4% and 7 haplotypes were produced. The length of cpDNA petB/petD sequence was 616 bp, of which 1 was variable sites with a percentage of 0.16% was found. The G+C content of the sequence was 34.6% and 2 haplotypes were found. The petB/petD region of L.pumilum was more conserved than ITS sequences and evolved more slowly. The present distribution range of L.pumilum has probably experienced range expansion by the haplotype analysis of mismatch distribution and neutral test, and AMOVA analysis showed that the genetic variation of L.pumilum mainly existed within populations. The N_ST >G_ST(P>0.01)further showed that there was no significant phylogeographic structure of genetic variation in L.pumilum. Therefore, the nrDNA ITS sequence of L.pumilum was more suitable for phylogeographic study of this species.

Key words: southeastern of Qinghai-Tibetan Plateau, Lilium pumilum, ITS, petB/petD, molecular evolution

中图分类号:

Q949.71+8.23

蒋福娟, 唐楠, 唐道城, 巨秀婷, 孙静清. 青藏高原东南部山丹nrDNA ITS和cpDNA petB/petD 序列的分子进化特点[J]. 植物研究, 2018, 38(1): 117-124.

JIANG Fu-Juan, TANG Nan, TANG Dao-Cheng, JU Xiu-Ting, SUN Jing-Qing. Characteristic of Molecular Evolution for Lilium pumilum Redouté on Southeast of the Qinghai-Tibetan Plateau using nrDNA ITS and cpDNA petB/petD Sequence Analysis[J]. Bulletin of Botanical Research, 2018, 38(1): 117-124.

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青藏高原东南部山丹nrDNA ITS和cpDNA petB/petD 序列的分子进化特点

Characteristic of Molecular Evolution for Lilium pumilum Redouté on Southeast of the Qinghai-Tibetan Plateau using nrDNA ITS and cpDNA petB/petD Sequence Analysis

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