内生真菌ZPRa-R-1对红景天中关键信号分子及主要次生代谢物的影响

doi:10.7525/j.issn.1673-5102.2016.03.015

植物研究 ›› 2016, Vol. 36 ›› Issue (3): 416-420.doi: 10.7525/j.issn.1673-5102.2016.03.015

内生真菌ZPRa-R-1对红景天中关键信号分子及主要次生代谢物的影响

王梦亮¹, 焦晋^1,2, 邢婕³, 田俊生³, 崔晋龙¹, 王俊宏¹

1. 山西大学应用化学研究所, 太原 030006;
2. 山西大学生物技术研究所, 太原 030006;
3. 山西大学中医药现代研究中心, 太原 030006

收稿日期:2015-09-29 出版日期:2016-05-15 发布日期:2016-05-20
通讯作者: 崔晋龙,E-mail:CJL717@163.com E-mail:CJL717@163.com
作者简介:王梦亮(1966-),男,教授,博士,主要从事生物转化及药物中间体的合成的研究。
基金资助:
国家自然科学基金(31270383);山西省自然科学基金(2014011029-1);高等学校博士学科点专项科研基金(20121401120001);林业公益性行业科研专项(201304326)

Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata

WANG Meng-Liang¹, JIAO Jin^1,2, XING Jie³, TIAN Jun-Sheng³, CUI Jin-Long¹, WANG Jun-Hong¹

1. Insititute of Applied Chemistry, Shanxi University, Taiyuan 030006;
2. Insititute of Biotechnology, Shanxi University, Taiyuan 030006;
3. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006

Received:2015-09-29 Online:2016-05-15 Published:2016-05-20

摘要/Abstract

摘要： 研究内生真菌ZPRa-R-1对大花红景天组培苗中信号分子NO,SA和H₂O₂,及主要次生代谢物酪醇(p-tyrosol)和红景天苷(salidroside)积累的影响。分别采用Greiss试剂法、RP-HPLC荧光法、硫酸钛法检测NO,SA和H₂O₂等信号分子;采用分光光度法检测关键酶PAL和CA4H的活性;采用RP-HPLC检测酪醇和红景天苷。ZPRa-R-1接种于红景天,首先引起宿主信号分子NO含量增高,其次是SA和H₂O₂,它们达到最大值的时间分别是共生后第8,10和12d,含量分别为0.193μmol·g^-1,0.062μg·g^-1,0.092μmol·g^-1;关键酶PAL和CA4H活性最大值分别为166.400U·g^-1和0.625U·g^-1·h^-1,分别是对照组的8和1.5倍,最终引起酪醇和红景天苷的积累效应,在第8和10d达到最大值,含量分别为8.656和0.498mg·g^-1,是对照组的7和2.8倍。研究结果表明内生真菌ZPRa-R-1能通过诱导激活宿主信号分子网络系统,引起关键酶活性的变化,进而调控酪醇和红景天苷等次生代谢物的积累。

关键词: 红景天, 内生真菌, 信号分子, 酪醇, 红景天苷

Abstract: We studied the effects on the key signal molecules and the main secondary metabolites's content in tissue culture of Rhodiola crenulata inoculated with endophic fungi ZPRa-R-1. We selected Greiss method, RP-HPLC and Titanium sulfate method to detect the contents of NO, SA and H₂O₂, respectively, and detected the key enzymes, PAL and CA4H by spectrophotometry. p-Tyrosol and salidroside in Rhodiola seedling co-cultured with fungi were evaluated by RP-HPLC. The content of NO increased firstly, followed by SA and H₂O₂ after that Rhodiola seedling inoculated with endophic fungi. The maximum contents of NO, SA and H₂O₂ were detected at 8, 10 and 12 d, and they were 0.193 μmol·g^-1, 0.062 μg·g^-1 and 0.092 μmol·g^-1, respectively. The maximum contents of PAL and CA4H were 166.400 U·g^-1, 0.625 U·g^-1·h^-1, and were 8 and 1.5 times as much as that of control, repectively. The contents of p-tyrosol and salidroside were accumulated, and the time of each of them reaching the maximum content was on 8 and 10 d, and the contents were 8.656 and 0.498 mg·g^-1, and were 7 and 2.5 times as much as that of control, respectively. Therefore, ZPRa-R-1 has the capacity of regulating signals and promoting the activities of PAL and CA4H, and finally promoted accumulation of p-tyrosol and salidroside in co-culturing seedling of fungi and Rhodiola.

Key words: Rhodiola crenulata, endophytic fungi, signal molecule, p-tyrosol, salidroside

中图分类号:

S567

王梦亮, 焦晋, 邢婕, 田俊生, 崔晋龙, 王俊宏. 内生真菌ZPRa-R-1对红景天中关键信号分子及主要次生代谢物的影响[J]. 植物研究, 2016, 36(3): 416-420.

WANG Meng-Liang, JIAO Jin, XING Jie, TIAN Jun-Sheng, CUI Jin-Long, WANG Jun-Hong. Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata[J]. Bulletin of Botanical Research, 2016, 36(3): 416-420.

参考文献

1. 中华人民共和国药典(2010一部)[S].北京:中国医药科技出版社,2010:144-144.
2. Khanum F,Bawa A S,Singh B. Rhodiola rosea:A versatile adaptogen[J].Comprehensive Reviews in Food Science and Food Safety,2005,4:55-62.
3. Stierle A,Strobel G,Stierle D. Taxol and taxane production by Taxomyces andreanae,an endophytic fungus of Pacific yew[J].Science,1993,260:214-216.
4. Dietrich A,Mayer J E,Hahlbrock K. Fungal elicitor triggers rapid,transient,and specific protein phosphorylation in Parsley cell suspension cultures[J].Journal of Biological Chemistry,1990,265:6360-6368.
5. Nurnberger T,Colling C,Hahlbrock K,et al. Perception and transduction of an elicitor signal in cultured Parsley cells[J].Biochemical Society Symposium,1994,60:173-182.
6. Li P Q,Yan M,Shan T J,et al. Effects of polysaccharide elicitors from endophytic Fusarium oxysporium Dzf17 on growth and diosgenin production in cell suspension culture of Dioscorea zingiberensis[J].Molecules,2011,16(11):9003-9008.
7. 陶金华,汪冬庚,濮雪莲,等. 一氧化氮和水杨酸依次介导内生真菌诱导子促进苍术细胞中苍术素生物合成的信号转导[J].中草药,2014,45(5):701-708.
8. Cui J L,Guo T T,Ren Z X,et al. Diversity and Antioxidant Activity of Culturable Endophytic Fungi from Alpine Plants of Rhodiola crenulata,R. angusta and R. sachalinensis[J].Plos One,2015,10(3):1-16.
9. 杜研. 诱导子对黄芪愈伤组织生长及代谢的影响[D].长春:吉林农业大学生命科学学院,2007.
10. Neill S J,Desikan R,Hancock J T. Nitric oxide signaling in plants[J].New Phytologist,2003,159:11-12.
11. Delledonne M,Zeier J,Marocco A,et al. Signal interaction between nitricoxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response[J].Proceedings of the National Academy of Science,USA,2001,98:13454-13459.
12. 徐茂军. 一氧化氮:植物细胞次生代谢信号转导网络可能的关键节点[J].自然科学进展,2007,17(12):1622-1630.
13. 刘颖,魏希颖. 内生真菌对植物次生代谢产物的转化[J].天然产物研究与开发,2014,26:300-303.
14. 方芳,戴传超,张波. 茅苍术悬浮细胞系建立及内生真菌诱导子对其挥发油积累的影响[J].中草药,2009,40(3):452-455.
15. Gao F K,Ren C G,Dai C C. Signaling Effects of Nitric Oxide,Salicylic Acid,and Reactive Oxygen Species on Isoeuphpekinensin Accumulation in Euphorbia pekinensis Suspension Cells Induced by an Endophytic Fungal Elicitor[J].Journal of Plant Growth Regulation,2012,31:490-497.

[1]	申晨静, 武文博, 耿露冉, 王福龙, 赵鹏舟, 宋金辉, 詹亚光, 尹静. 水杨酸、纳米氧化锌和促生真菌YZ13-1在水曲柳抵御干旱胁迫中的调控作用[J]. 植物研究, 2023, 43(3): 388-395.
[2]	张雨, 苏旭, 刘玉萍, 刘涛, 郑长远, 苏丹丹, 王亚男, 吕婷. 喜马红景天叶绿体基因组特征及其系统发育分析[J]. 植物研究, 2022, 42(4): 602-612.
[3]	雷淑芸1,2；高庆波1；付鹏程1；杨慧玲1；陈世龙1；张发起1,3. 基于Solexa高通量测序的唐古特红景天（Rhodiola algida*）微卫星信息分析[J]. 植物研究, 2014, 34(6): 829-834.
[4]	刘威;袁晓婷;张艳艳;闫永庆*. 胭脂红景天引种至西藏日喀则其渗透调节物质及保护酶活性的变化[J]. 植物研究, 2013, 33(6): 697-700.
[5]	吴连杰;荆元芳;任安芝*;高玉葆. 感染内生真菌的羊草对克氏针茅种子萌发的影响[J]. 植物研究, 2012, 32(6): 669-674.
[6]	郑维列;田大伦;卢杰*;罗建. 高寒植物长鞭红景天种群结构及数量特征[J]. 植物研究, 2009, 29(4): 402-410.
[7]	林枫;李川;张欣;包晓影;任安芝;高玉葆*. 内生真菌感染对3个不同地理种群羽茅光合特性的影响[J]. 植物研究, 2009, 29(1): 61-68.
[8]	王化田, 祖元刚, 毛子军. 超临界CO₂萃取红景天中红景天苷、苷元酪醇的研究[J]. 植物研究, 2004, 24(4): 462-465.
[9]	王桂芹, 赵岩, 段亚军. 高山红景天解剖学研究[J]. 植物研究, 2004, 24(1): 93-95.
[10]	谷会岩, 宫立冬, 马玲. 高效毛细管电泳法测定高山红景天中红景天甙含量的比较研究[J]. 植物研究, 2002, 22(3): 337-340.
[11]	吴双秀, 尚辛亥, 戴绍军, 阎秀峰. 高山红景天年龄与根部红景天甙含量的关系[J]. 植物研究, 2001, 21(2): 252-257.
[12]	周福军, 颜庭芬, 王琴, 阎秀峰, 祖元刚. 高山红景天形态分化与生存环境关系的研究[J]. 植物研究, 2001, 21(1): 90-96.
[13]	王洋, 张璞, 于涛, 王化田, 阎秀峰. 高效液相色谱法测定红景天甙含量方法的研究[J]. 植物研究, 2001, 21(1): 113-115.
[14]	阎秀峰, 王洋, 杨意, 周福军, 尚辛亥. 大海林地区不同生境高山红景天根部红景天甙含量的差异[J]. 植物研究, 2000, 20(2): 173-179.
[15]	阎秀峰, 王玉杰, 王洋, 孙海芹, 祖元刚. 长白山高山红景天的气体交换特性[J]. 植物研究, 1999, 19(3): 273-280.

内生真菌ZPRa-R-1对红景天中关键信号分子及主要次生代谢物的影响

Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价