7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析

doi:10.7525/j.issn.1673-5102.2012.02.014

植物研究 ›› 2012, Vol. 32 ›› Issue (2): 208-212.doi: 10.7525/j.issn.1673-5102.2012.02.014

7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析

陈燕英;黎云祥^*;权秋梅;廖兴利;成仙鸽

西华师范大学西南野生动植物资源保护教育部重点实验室,南充　637002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-03-20 发布日期:2012-03-20
通讯作者: 黎云祥
基金资助:

Genetic Diversity and Relationship Among 7 Species of Genus Epimedium in Sichuan Revealed by ISSR Analysis

CHEN Yan-Ying;LI Yun-Xiang*;QUAN Qiu-Mei;LIAO Xing-Li;CHENG Xian-Ge

Key Laboratory of South West China Wild Life Resources Conservation,China West Normal University,Nanchong　637002

Received:1900-01-01 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20
Contact: LI Yun-Xiang
Supported by:

摘要/Abstract

摘要： 利用ISSR分子标记对7种12居群的川产淫羊藿属植物进行遗传多样性及亲缘关系分析。结果发现23个ISSR随机引物共扩增194条清晰条带，其中169条具多态性，平均多态性位点比率为87.11%，有效等位基因数Ne=1.534 2，Nei基因多样性指数H=0.314 4，Shannon多样性指数I=0.469 7，表明物种间遗传多样性丰富。7种川产淫羊藿属植物间的遗传相似系数为0.653 2～0.748 9，聚类分析和主成分分析直观的显示出12份供试材料间的亲缘关系，并将其分为两类。表明ISSR分子标记技术可以用于川产淫羊藿属植物的遗传多样性和亲缘关系分析。

关键词: 淫羊藿属, ISSR, 遗传多样性, 亲缘关系

Abstract: In order to investigate the genetic diversity and relationship of genus Epimedium in Sichuan province, 7 species including 12 populations were used to study the genetic variation by the ISSR marker technique. The result showed that 194 DNA fragments among 12 populations were amplified using 23 reliable ISSR primers, among which 169 DNA bands were polymorphic (the percentage of polymorphic bands reached 87.11%). The genetic distance and similarity among 12 populations were 0.298 0-0.620 7 and 0.653 2-0.748 9, indicating variation of genetic diversity existed among different populations. The observed effective number of alleles (Ne), Nei’s gene diversity index (He) and Shannon’s information index (I) were 1.534 2, 0.314 4 and 0.469 7, respectively, showing the geographical distribution was very obvious. The result of cluster analysis using the unweighted pairgroup method with arithmetic average (UPGMA) and Principal Component analysis (PCA) based on ISSR data both showed that these tested resources were divided into two main groups. In addition, the ISSR marker technique is suitable for the analysis of the genetic diversity and relationship of genus Epimedium in Sichuan.

Key words: Epimedium, ISSR, genetic diversity, genetic relationship

中图分类号:

S567.2

陈燕英;黎云祥*;权秋梅;廖兴利;成仙鸽. 7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析[J]. 植物研究, 2012, 32(2): 208-212.

CHEN Yan-Ying;LI Yun-Xiang*;QUAN Qiu-Mei;LIAO Xing-Li;CHENG Xian-Ge. Genetic Diversity and Relationship Among 7 Species of Genus Epimedium in Sichuan Revealed by ISSR Analysis[J]. Bulletin of Botanical Research, 2012, 32(2): 208-212.

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7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析

Genetic Diversity and Relationship Among 7 Species of Genus Epimedium in Sichuan Revealed by ISSR Analysis

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