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植物研究 ›› 2010, Vol. 30 ›› Issue (6): 668-673.doi: 10.7525/j.issn.1673-5102.2010.06.008

• 论文 • 上一篇    下一篇

油松成熟胚培养直接器官发生与植株再生

孔冬梅;万婷   

  1. 山西大学生命科学学院,太原 030006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:
     

Direct Organogenesis and Plantlet Regeneration from Mature Zygotic Embryos of Chinese Pine(Pinus tabulaeformis Carr.)

KONG Dong-Mei;WAN Ting   

  1. School of Life Science,Shanxi University,Taiyuan 030006
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Supported by:
     

摘要: 以油松(Pinus tabulaeformis Carr.)成熟合子胚为外植体进行了直接器官发生研究。在添加1~5 mg·L-1 BA,或再配合添加1~10 mg·L-1 2,4-D的DCR和MS培养基上均能诱导发生不定芽。在只添加1 mg·L-1 BA的DCR培养基上不定芽诱导率最高,为90%。及时将不定芽继代于不含生长调节物质的DCR培养基上有利于芽的伸长。添加1 mg·L-1 IBA的1/2DCR培养基最适于不定根的诱导,在该培养基上不定根诱导率达到47.1%。再生植株在蛭石基质上炼苗后转到温室培养,成活率高达86.4%,且生长正常。

关键词: 油松, 微繁, 不定芽, 生根, 炼苗

Abstract: Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis. Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg·L-1 benzyladenine (BA) with or without 1~10 mg·L-1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction frequency of adventitious buds (90%) was obtained on DCR medium containing 1 mg·L-1 BA. Subsequent transfer of buds to plant growth regulator (PGR)-free medium in time was advantageous for elongation of adventitious buds. 1/2DCR medium with 1 mg·L-1 indole-3-butyric acid (IBA) was suitable for adventitious root initiation, on which average rooting frequency was 47.1%. Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse, where they had a survival rate of 86.4% and no morphological abnormalities.

Key words: Chinese pine, micropropagation, adventitious bud, rooting, acclimatization

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