关键词: 乌药, 遗传多样性, 遗传分化, ISSR

Abstract: Eight populations of Lindera aggregata were collected from Zhejiang Province and the genetic diversity and genetic differentiation were analyzed by using inter-simple sequence repeat(ISSR) molecular makers. 12 relaible primers were selected from total 100 primers and 125 loci were detected in 160 individuals. 100 loci were polymorphic and the percentage of polymorphic loci(P) at species level was 80% with an average of 60.5% at population level. The genetic diversity estimated by Shannon information index(I) and Nei’s gene index(h) was 0.376 2 and 0.246 4, respectively, while those at population level averaged 0.317 3 and 0.213 1, respectively. Analysis of molecular variance(ANOVA) showed that only 11.44% of the total variation was distributed among populations, while 88.56% was distributed among individuals within population. The gene differentiation coefficient(G_st) and the gene flow(N_m) was 0.134 9 and 3.205 2, respectively. The pair-wise genetic distance averaged 0.049 4. By using the unweighted pair group method with arithmetic mean(UPGMA), the eight populations could be divided into two groups. Shuimokeng, Shangpan and Cangshan populations collected in Taizhou city were clustered into one group and the other five populations were clustered into the other group.

Key words: Lindera aggregate, genetic diversity, genetic differentiation, ISSR