In majority of plants, colorful pollen is rarely seen on the surface of pollen except light yellow. In this article, the existence of blue pollen was discovered in Caryopteris incana, and the color of anther and pollen at different developmental stages was observed by microscope, and the anthocyanin components of mature pollen were analyzed by UPLC/MS(Ultra Performance Liquid Chromatography-tandem mass spectrometry). The results showed that mature anthers and pollens were blue due to the rich anthocyanins. The color of anther and pollen changed obviously at young buds, early flowering buds and flowering stages, which were light yellow, light blue and blue purple respectively. The blue pigments of pollen mainly existed in the surface of pollen coat and tapetal cells by the cross-section observation. The UPLC/MS analysis showed that there were six kinds of anthocyanins on the pollen surface at the flowering stage, among which the main blue components were Delphinidin 3-O-glucoside and Malvidin 3,5-O-diglucoside, followed by Peonidin, Cyanidin and Petunidin, while the content of Pelargonidin was the lowest.