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    Observation on the Formation and Composition Analysis of Blue Pollen of Caryopterisincana
    Qinghua YANG, Binjie GE, Dasheng ZHANG
    Bulletin of Botanical Research    2023, 43 (5): 794-800.   DOI: 10.7525/j.issn.1673-5102.2023.05.016
    Abstract769)   HTML8)    PDF(pc) (2669KB)(76)       Save

    In majority of plants, colorful pollen is rarely seen on the surface of pollen except light yellow. In this article, the existence of blue pollen was discovered in Caryopteris incana, and the color of anther and pollen at different developmental stages was observed by microscope, and the anthocyanin components of mature pollen were analyzed by UPLC/MS(Ultra Performance Liquid Chromatography-tandem mass spectrometry). The results showed that mature anthers and pollens were blue due to the rich anthocyanins. The color of anther and pollen changed obviously at young buds, early flowering buds and flowering stages, which were light yellow, light blue and blue purple respectively. The blue pigments of pollen mainly existed in the surface of pollen coat and tapetal cells by the cross-section observation. The UPLC/MS analysis showed that there were six kinds of anthocyanins on the pollen surface at the flowering stage, among which the main blue components were Delphinidin 3-O-glucoside and Malvidin 3,5-O-diglucoside, followed by Peonidin, Cyanidin and Petunidin, while the content of Pelargonidin was the lowest.

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    Flowering Biological Characteristics and Breeding System of Lumnitzera racemosa
    Yanqing CAI, Yujun CHEN, Mei LI, Chuangfa DENG, Liejian HUANG
    Bulletin of Botanical Research    2024, 44 (1): 152-160.   DOI: 10.7525/j.issn.1673-5102.2024.01.017
    Abstract71)   HTML7)    PDF(pc) (2518KB)(105)       Save

    In order to reveal the endangered mechanism and carry out related conservation actions of Lumnitzera racemosa, we observed the flowering dynamics of the introduced L. racemosa in Dangan Island Provincial Nature Reserve, Zhuhai, Guangdong, and investigated the breeding system of L. racemosa by using the out-crossing index(OCI), pollen-ovule ratio(P/O), pollen viability and stigma receptivity, and artificial pollination. The results showed that: (1)The flowering period of L. racemosa population started May 16, the full blooming period was from May 25 to July 1, and the last flowering period was from July 5 to August 31, and the flowering period of L. racemosa population was up to 106 days. The flowering duration of inflorescence was 5-18 days, and average duration was (10.80±3.58) days; The single flowering period of L. racemose lasted 7-13 days, with an average of (9.1±1.3) days. (2)Pollen viability was highest on the day of flowering, reaching 58.73%, and decreased on the second day and no longer viable was on the third day; Stigma receptivity was weakest at the first day of opening, then gradually strengthened, and was strongest at 100% at the third day, and maturity time of male and female was inconsistent. (3)OCI was 4, the breeding system of L. racemosa was mainly outcrossing with partial self-compatible, which required pollinator; P/O was 5 740.50±1 318.20, the breeding system was obligate xenogamy; pollination test showed that the breeding system was a mixed mating system with both self and xenogamy. Based on the above, the breeding system of L. racemosa was mainly outcrossing with partial self-compatibility and requiredr pollinators.

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    Embryo Development and Features of Seed and Fruit of Endangered Plant Cymbidium goeringii var. longibracteatum
    Renrui WANG, Xin LIU, Jie LI
    Bulletin of Botanical Research    2023, 43 (6): 953-960.   DOI: 10.7525/j.issn.1673-5102.2023.06.017
    Abstract70)   HTML6)    PDF(pc) (4479KB)(148)       Save

    The embryo development, seed formation and fruit anatomical characteristics of Cymbidium goeringii var. longibracteatum were investigated by microscopic and ultrastructure obsevation on the capsules at various developmental stages obtained by artificial self-pollination. The results showed that: megasporocyte and mature embryo sac were observed at 60 d and 80 d after pollination resepectively, and the female gametophyte was of polygonum type. Spherical embryo and suspensor structures formed after 120 d, and the suspensor degenerated and embryo body was at the globular stage after 180 d, and development of the embryo was of the solanad type. The seeds were extremely tiny, consisting of endopleura, episperm and undifferentiated globular embryo, accounting for about 24% of the seed embryo volume, and with little content of nutrients, and an air cavity between the seed coats.The ovary was composed of six carpel valves, three of which were fertile with a ‘V’ shaped placenta, and the others were infertile carpel valves without placenta. The average weight of a single seed was (3.92 ±0.40) μg, and the number of seeds in a capsule was(37.98±3.71)×104.

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